PGAP1 Polyclonal antibody

PGAP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 55392-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

Bst1, GPI inositol deacylase, hPGAP1, ISPD3024, PGAP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inhuman testis tissue, HEK-293 cells, MCF-7 cells, mouse testis tissue
Positive IHC detected inhuman testis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55392-1-AP targets PGAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 post-GPI attachment to proteins 1
别名 Bst1, GPI inositol deacylase, hPGAP1, ISPD3024, PGAP1
计算分子量 105 kDa
观测分子量 49-53 kDa, 85-90 kDa
GenBank蛋白编号NM_024989
基因名称 PGAP1
Gene ID (NCBI) 80055
RRIDAB_11232412
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ75T13
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PGAP1 belongs to the GPI inositol-deacylase family. It is involved in inositol deacylation of GPI-anchored proteins. GPI inositol deacylation may important for efficient transport of GPI-anchored proteins from the endoplasmic reticulum to the Golgi. PGAP1 is the GPI inositol-deacylase (PMID:14734546). This antibody is specific to PGAP1.

实验方案

Product Specific Protocols
WB protocol for PGAP1 antibody 55392-1-APDownload protocol
IHC protocol for PGAP1 antibody 55392-1-APDownload protocol
IF protocol for PGAP1 antibody 55392-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
HumanIHC

Nature

A single-cell atlas of human and mouse white adipose tissue.

Authors - Margo P Emont
humanWB

Mol Cell

Integrative omics indicate FMRP sequesters mRNA from translation and deadenylation in human neuronal cells

Authors - Tatsuaki Kurosaki
humanWB

Cancer Med

Identification of novel pQTL-SNPs associated with lung adenocarcinoma risk: A multi-stage study

Authors - Yutong Wu
humanWB

J Biol Chem

Identification of mammalian glycoproteins with type-I LacdiNAc structures synthesized by the glycosyltransferase B3GALNT2.

Authors - Takahiro Nakane
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