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验证数据展示

  • WB analysis using 28445-1-AP (same clone as 28445-1-PBS)

    WB analysis using 28445-1-AP (same clone as 28445-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28445-1-AP (PGAM5 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28445-1-PBS in a different storage buffer formulation.

  • WB analysis of LNCaP using 28445-1-AP (same clone as 28445-1-PBS)

    WB analysis of LNCaP using 28445-1-AP (same clone as 28445-1-PBS)

    WB result of PGAM5 antibody (28445-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PGAM5 transfected LNCaP cells. This data was developed using the same antibody clone with 28445-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 28445-1-AP (same clone as 28445-1-PBS)

    IF Staining of MCF-7 using 28445-1-AP (same clone as 28445-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PGAM5 antibody (28445-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28445-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 28445-1-AP (same clone as 28445-1-PBS)

    IHC staining of human lung cancer using 28445-1-AP (same clone as 28445-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28445-1-AP (PGAM5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28445-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 28445-1-AP (same clone as 28445-1-PBS)

    IHC staining of human lung cancer using 28445-1-AP (same clone as 28445-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28445-1-AP (PGAM5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28445-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PGAM5 Polyclonal antibody, PBS Only

PGAM5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 28445-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Bcl-XL-binding protein v68, BXLBv68, EC:3.1.3.16, Serine/threonine-protein phosphatase PGAM5, mitochondrial

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

28445-1-PBS targets PGAM5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag28195

Product name: Recombinant human PGAM5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-255 aa of BC008196

Sequence: MAFRQALQLAACGLAGGSAAVLFSAVAVGKPRAGGDAEPRPAEPPAWAGGARPGPGVWDPNWDRREPLSLINVRKRNVESGEEELASKLDHYKAKATRHIFLIRHSQYHVDGSLEKDRTLTPLGREQAELTGLRLASLGLKFNKIVHSSMTRAIETTDIISRHLPGVCKVSTDLLREGAPIEPDPPVSHWKPEAVQYYEDGARIEAAFRNYIHRADARQEEDSYEIFICHANVIRYIVCSIPPLLSAGDFVVLGS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphoglycerate mutase family member 5
别名 Bcl-XL-binding protein v68, BXLBv68, EC:3.1.3.16, Serine/threonine-protein phosphatase PGAM5, mitochondrial
计算分子量 32 kDa
观测分子量32 kDa
GenBank蛋白编号BC008196
基因名称 PGAM5
Gene ID (NCBI) 192111
RRIDAB_2881143
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96HS1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Phosphoglycerate mutase 5 (PGAM5) is a mitochondrial Serine (Ser)/Threonine (Thr) phosphatase normally located in the inner mitochondrial membrane. Upon mitochondrial dysfunction, PGAM5 recruits and dephosphorylates Drp1 at Ser-637, triggers its GTPase activity and promotes mitochondrial fission. PGAM5 regulates mitophagy by stabilizing PINK1 under stress conditions, which recruits E3 ubiquitin ligase PARKIN for degradation of the damaged mitochondria. PGAM5 can be cleaved and released to the cytoplasm through PARKIN, which activates Wnt signaling and induces mitochondrial biogenesis (PMID: 32439975). PGAM5 has 2 isoforms with the molecular mass of 28 and 32 kDa.

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