验证数据展示
经过测试的应用
| Positive WB detected in | pig stomach tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33764-1-AP targets PGA4 in WB, ELISA applications and shows reactivity with human, pig samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, pig |
| 免疫原 |
CatNo: Ag37827 Product name: Recombinant human PGA4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 111-261 aa of NM_001079808.3 Sequence: ACTNHNRFNPEDSSTYQSTSETVSITYGTGSMTGILGYDTVQVGGISDTNQIFGLSETEPGSFLYYAPFDGILGLAYPSISSSGATPVFDNIWNQGLVSQDLFSVYLSADDQSGSVVIFGGIDSSYYTGSLNWVPVTVEGYWQITVDSITM 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pepsinogen 4, group I (pepsinogen A) |
| 别名 | EC:3.4.23.1, Pepsin A-4, Pepsinogen-4 |
| 计算分子量 | 42kDa，388aa |
| 观测分子量 | 42-45 kDa |
| GenBank蛋白编号 | NM_001079808.3 |
| 基因名称 | PGA4 |
| Gene ID (NCBI) | 643847 |
| RRID | AB_3743072 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | P0DJD7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PGA4(Pepsinogen A4), also known as pepsinogen A, is a member of Peptidase A1 family. PGA4 is the precursor protein of digestive enzyme Pepsin, which is synthesized and secreted by the chief cells of gastric mucosa. Autocatalytic cleavage occurs under acidic conditions (pH 1-3), and about 41-44 amino acid fragments at the N-terminal are removed to form active pepsin. Serum pepsinogen level can be used as an important biomarker: screening index of atrophic gastritis, early diagnosis and screening of gastric cancer. The ratio (PGR) of pepsinogen I(PGI, mainly PGA) to pepsinogen II(PGII) is an important parameter to evaluate the function of gastric mucosa. The observed molecular weight is 42-45 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PGA4 antibody 33764-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
