验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, MCF-7 cells, SH-SY5Y cells, fetal human brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
13783-1-AP targets PFN2 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | rat |
| 免疫原 | PFN2 fusion protein Ag4792 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | profilin 2 |
| 别名 | Prifilin 2, D3S1319E, PFL, profilin 2, Profilin II |
| 计算分子量 | 140 aa, 15 kDa |
| 观测分子量 | 14-15 kDa |
| GenBank蛋白编号 | BC018049 |
| 基因名称 | PFN2 |
| Gene ID (NCBI) | 5217 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P35080 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PFN2 (profilin 2) is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. The MW of this protein is 15 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PFN2 antibody 13783-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |