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PFKP Monoclonal antibody

PFKP Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit and More (1)

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H4D4

Cat No : 68129-1-Ig

Print datasheet

Synonyms

6 phosphofructokinase type C, FLJ40226, PFK C, PFKF, PFKP, Phosphofructokinase 1, phosphofructokinase, platelet, Phosphohexokinase

验证数据展示

  • WB analysis using 68129-1-Ig

    WB analysis using 68129-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68129-1-Ig (PFKP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IF Staining of HeLa using 68129-1-Ig

    IF Staining of HeLa using 68129-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PFKP antibody (68129-1-Ig, Clone: 1H4D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inLNCaP cells, rabbit brain tissue, rat brain tissue, mouse brain tissue, A549 cells, MCF-7 cells, HEK-293 cells, HeLa cells, Jurkat cells
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

68129-1-Ig targets PFKP in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human, Mouse, Rat, Rabbit
Cited Reactivityhuman
Immunogen PFKP fusion protein Ag25206 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name phosphofructokinase, platelet
Synonyms 6 phosphofructokinase type C, FLJ40226, PFK C, PFKF, PFKP, Phosphofructokinase 1, phosphofructokinase, platelet, Phosphohexokinase
Calculated Molecular Weight 784 aa, 86 kDa
Observed Molecular Weight80-86 kDa
GenBank Accession NumberBC029138
Gene Symbol PFKP
Gene ID (NCBI) 5214
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ01813
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PFKP(6-phosphofructokinase, platelet type) is also named as PFKF, PFK-C and belongs to the phosphofructokinase family. This form of PFK is best called the 'platelet' type and symbolized PFKP because it is the only form made by platelets, whereas fibroblasts have more than one form of PFK (Francke (1983)). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The observed molecular weight of PFKP monomer is 80-86 kDa. The molecular mass of PFKP dimer is 171 kDa (PMID: 28607489).

实验方案

Product Specific Protocols
WB protocol for PFKP antibody 68129-1-IgDownload protocol
IF protocol for PFKP antibody 68129-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

medRxiv

Depressed Proximal Glycolysis in Myocardium Of Human Heart Failure with Preserved Ejection Fraction

Authors - Navid Koleini
View Article