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验证数据展示

  • WB analysis using RMX00037

    WB analysis using RMX00037

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00037 (PFKM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using RMX00037

    IHC staining of mouse skeletal muscle using RMX00037

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using RMX00037 (PFKM antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PFKM antibody included
  • IF Staining of HeLa using RMX00037

    IF Staining of HeLa using RMX00037

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PFKM antibody (RMX00037) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Multi-rAb® PFKM Multi-Recombinant antibody

PFKM Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. RMX00037

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

6-phosphofructokinase type A, ATP-dependent 6-phosphofructokinase, muscle type, ATP-PFK, EC:2.7.1.11, PFK A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, L02 cells, Raji cells, mouse liver tissue, rat liver tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PFKM Ready-To-Use IHC Kit. PFKM primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

RMX00037 targets PFKM in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Recombinant protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Multi-Recombinant
产品类型 Antibody
全称 phosphofructokinase, muscle
别名 6-phosphofructokinase type A, ATP-dependent 6-phosphofructokinase, muscle type, ATP-PFK, EC:2.7.1.11, PFK A
观测分子量 75-85 kDa
GenBank蛋白编号BC021203
基因名称 PFKM
Gene ID (NCBI) 5213
RRIDAB_3750091
偶联类型 Unconjugated
形式Liquid
纯化方式N/A
UNIPROT IDP08237
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PFKM, also named as GSD7, PFK-1, PFK-M and PFKX, belongs to the phosphofructokinase family and two domains subfamily. PFKM catalyzes the reaction: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. It is a key regulatory enzyme in glycolysis. Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7). PFKM has three isoforms with molecular masses of 85, 82 and 93 kDa.

实验方案

Product Specific Protocols
IF protocol for PFKM antibody RMX00037Download protocol
IHC protocol for PFKM antibody RMX00037Download protocol
WB protocol for PFKM antibody RMX00037Download protocol
Standard Protocols
Click here to view our Standard Protocols
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