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验证数据展示

  • WB analysis using 84281-5-RR

    WB analysis using 84281-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84281-5-RR (PFKM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 84281-5-RR

    IHC staining of mouse skeletal muscle using 84281-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84281-5-RR (PFKM antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PFKM antibody included
  • IF Staining of HeLa using 84281-5-RR

    IF Staining of HeLa using 84281-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PFKM antibody (84281-5-RR, Clone: 241598B2 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

  • Affinity and Kinetic Characterization of 84281-5-RR

    Affinity and Kinetic Characterization of 84281-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84281-5-RR against Human PFKM were performed. The affinity constant is 3.01 nM.

PFKM Recombinant antibody

PFKM Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84281-5-RR

产品说明书

CloneNo. 241598B2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

PFK A, EC:2.7.1.11, ATP-PFK, ATP-dependent 6-phosphofructokinase, muscle type, 6-phosphofructokinase type A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse skeletal muscle tissue, PC-3 cells, Raji cells
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PFKM Ready-To-Use IHC Kit. PFKM primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84281-5-RR targets PFKM in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 PFKM fusion protein Ag30840 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 phosphofructokinase, muscle
别名 PFK A, EC:2.7.1.11, ATP-PFK, ATP-dependent 6-phosphofructokinase, muscle type, 6-phosphofructokinase type A
观测分子量 75-85 kDa
GenBank蛋白编号BC021203
基因名称 PFKM
Gene ID (NCBI) 5213
RRIDAB_3671827
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08237
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PFKM, also named as GSD7, PFK-1, PFK-M and PFKX, belongs to the phosphofructokinase family and two domains subfamily. PFKM catalyzes the reaction: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. It is a key regulatory enzyme in glycolysis. Defects in PFKM cause glycogen storage disease type 7 (GSD7). PFKM has three isoforms with molecular weights of 85, 82, and 93 kDa.

实验方案

Product Specific Protocols
WB protocol for PFKM antibody 84281-5-RRDownload protocol
IHC protocol for PFKM antibody 84281-5-RRDownload protocol
IF protocol for PFKM antibody 84281-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols