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验证数据展示

  • WB analysis using 68385-1-Ig (same clone as 68385-1-PBS)

    WB analysis using 68385-1-Ig (same clone as 68385-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68385-1-Ig (PFKL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68385-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 68385-1-Ig (same clone as 68385-1-PBS)

    IHC staining of human lung cancer using 68385-1-Ig (same clone as 68385-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68385-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PFKL antibody included
  • IHC staining of human lung cancer using 68385-1-Ig (same clone as 68385-1-PBS)

    IHC staining of human lung cancer using 68385-1-Ig (same clone as 68385-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68385-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PFKL antibody included
  • IF Staining of HuH-7 using 68385-1-Ig (same clone as 68385-1-PBS)

    IF Staining of HuH-7 using 68385-1-Ig (same clone as 68385-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HuH-7 cells using PFKL antibody (68385-1-Ig, Clone: 1H2G2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68385-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 68385-1-Ig (same clone as 68385-1-PBS)

    FC experiment of HEK-293 using 68385-1-Ig (same clone as 68385-1-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug PFKL Monoclonal antibody (68385-1-Ig, Clone:1H2G2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 68385-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 68385-1-Ig (same clone as 68385-1-PBS)

    IHC staining of mouse liver using 68385-1-Ig (same clone as 68385-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68385-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PFKL antibody included
  • IHC staining of mouse liver using 68385-1-Ig (same clone as 68385-1-PBS)

    IHC staining of mouse liver using 68385-1-Ig (same clone as 68385-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68385-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PFKL antibody included
  • IF Staining of HuH-7 using 68385-1-Ig (same clone as 68385-1-PBS)

    IF Staining of HuH-7 using 68385-1-Ig (same clone as 68385-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using PFKL antibody (68385-1-Ig, Clone: 1H2G2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68385-1-PBS in a different storage buffer formulation.

PFKL Monoclonal antibody, PBS Only

PFKL Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 68385-1-PBS

产品说明书

CloneNo. 1H2G2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

1H2G2, 6-phosphofructokinase type B, ATP-dependent 6-phosphofructokinase, liver type, ATP-PFK, EC:2.7.1.11

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PFKL Ready-To-Use IHC Kit. PFKL primary antibody included.

产品信息

68385-1-PBS targets PFKL in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag8250

Product name: Recombinant human PFKL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 424-780 aa of BC008964

Sequence: TGISHGHTVYVVHDGFEGLAKGQVQEVGWHDVAGWLGRGGSMLGTKRTLPKGQLESIVENIRIYGIHALLVVGGFEAYEGVLQLVEARGRYEELCIVMCVIPATISNNVPGTDFSLGSDTAVNAAMESCDRIKQSASGTKRRVFIVETMGGYCGYLATVTGIAVGADAAYVFEDPFNIHDLKVNVEHMTEKMKTDIQRGLVLRNEKCHDYYTTEFLYNLYSSEGNGVFDCRTNVLGHLQQGGAPTPFDRNYGTKLGVKAMLWLSEKLREVYRKGRVFANAPDSACVIGLKKKAAAFSPVTELKKDTDFEHRMPREQWWLSLRLMLKMLAQYRISMAAYVSGELEHVTRRTLSMDKGF

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphofructokinase, liver
别名 1H2G2, 6-phosphofructokinase type B, ATP-dependent 6-phosphofructokinase, liver type, ATP-PFK, EC:2.7.1.11
计算分子量 85 kDa
观测分子量 85 kDa
GenBank蛋白编号BC008964
基因名称 PFKL
Gene ID (NCBI) 5211
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP17858
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PFKL (6-phosphofructokinase, liver type) is also named as PFK-B and belongs to the phosphofructokinase family. The PFKL gene encodes the liver isoform of phosphofructokinase (PFK) (ATP: D-fructose-6-phosphate-1-phosphotransferase). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. In human beings, PFK exists as a system of three subunits: these are the muscle(PFKM), liver(PFKL), and platelet(PFKP) PFKs (PMID: 2139864). It has 2 isoforms produced by alternative splicing.

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