验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, MDA-MB-231 cells, PC-3 cells, Raji cells |
| Positive IHC detected in | human kidney tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HUVEC cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
29902-1-AP targets PFKFB4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag30571 Product name: Recombinant human PFKFB4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 139-232 aa of NM_001317134 Sequence: ATIFNFGEQNGYKTFFVESICVDPEVIAANIVQVKLGSPDYVNRDSDEATEDFMRRIECYENSYESLDEDLDRDLSYIKIMDVGQSYVVNRVAD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 |
| 别名 | 6PF 2 K/Fru 2,6 P2ase 4, PFK/FBPase 4 |
| 计算分子量 | 54 kDa |
| 观测分子量 | 54 kDa |
| GenBank蛋白编号 | NM_001317134 |
| 基因名称 | PFKFB4 |
| Gene ID (NCBI) | 5210 |
| RRID | AB_2935486 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16877 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PFKFB4 (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4), is a bi-function enzyme. On one hand, PFKFB4 synthesizes fructose 2,6-bisphosphate (F2,6-BP) to stimulate glycolysis, playing important roles in metabolic processes. On the other hand, PFKFB4 acts as a phosphatase to hydrolyze F2,6-BP into fructose-6-phosphate (F6P) and inorganic phosphate (Pi), functioning as a protein kinase to phosphorylate protein substrates. The dual function of PFKFB4 makes it involved in multiple biological processes such as cell cycle regulation, cell proliferation and transcriptional regulation(PMID: 33593309). PFKFB4 has been reported to be involved in a variety of cancers including human bladder cancer, gastric cancer, colon cancer, breast cancer and other malignant tumors(PMID: 21396842, PMID: 33732372, PMID: 31244553).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for PFKFB4 antibody 29902-1-AP | Download protocol |
| IHC protocol for PFKFB4 antibody 29902-1-AP | Download protocol |
| WB protocol for PFKFB4 antibody 29902-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
