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验证数据展示

  • WB analysis using 16045-1-AP (same clone as 16045-1-PBS)

    WB analysis using 16045-1-AP (same clone as 16045-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16045-1-AP (PFDN4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16045-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 16045-1-AP (same clone as 16045-1-PBS)

    IHC staining of human breast cancer using 16045-1-AP (same clone as 16045-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16045-1-AP (PFDN4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16045-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PFDN4 antibody included
  • IHC staining of human breast cancer using 16045-1-AP (same clone as 16045-1-PBS)

    IHC staining of human breast cancer using 16045-1-AP (same clone as 16045-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16045-1-AP (PFDN4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16045-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PFDN4 antibody included

PFDN4 Polyclonal antibody, PBS Only

PFDN4 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 16045-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PFDN4 Ready-To-Use IHC Kit. PFDN4 primary antibody included.

产品信息

16045-1-PBS targets PFDN4 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag8938

Product name: Recombinant human PFDN4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-134 aa of BC010953

Sequence: MAATMKKAAAEDVNVTFEDQQKINKFARNTSRITELKEEIEVKKKQLQNLEDACDDIMLADDDCLMIPYQIGDVFISHSQEETQEMLEEAKKNLQEEIDALESRVESIQRVLADLKVQLYAKFGSNINLEADES

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 prefoldin subunit 4
别名
计算分子量 134 aa, 15 kDa
观测分子量 15-20 kDa
GenBank蛋白编号BC010953
基因名称 PFDN4
Gene ID (NCBI) 5203
RRIDAB_2162573
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQP4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The human PFDN4, prefoldin 4, is a subunit of the heterohexameric chaperone protein and belongs to the prefoldin family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. Previous reports have shown that PFDN4 is upregulated in breast cancer cell lines and tumor tissues (PMID: 11381030; PMID: 9630229). PFDN4 expression may be a prognostic factor in colorectal cancer (PMID: 20552408).

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