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PFDN2 Polyclonal antibody

PFDN2 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 13053-1-AP

Print datasheet

Synonyms

PFD2, PFDN2, prefoldin subunit 2

验证数据展示

  • WB analysis of L02 using 13053-1-AP

    WB analysis of L02 using 13053-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 13053-1-AP (PFDN2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 13053-1-AP

    IHC staining of human lung cancer using 13053-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 13053-1-AP (PFDN2 antibody) at dilution of 1:100 (under 10x lens).

  • IF Staining of L02 using 13053-1-AP

    IF Staining of L02 using 13053-1-AP

    Immunofluorescent analysis of L02 cells using 13053-1-AP (PFDN2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inL02 cells, Neuro-2a cells
Positive IHC detected inhuman lung cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inL02 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13053-1-AP targets PFDN2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, WB,ELISA
Tested Reactivity human, mouse
Immunogen PFDN2 fusion protein Ag3720 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name prefoldin subunit 2
Synonyms PFD2, PFDN2, prefoldin subunit 2
Calculated Molecular Weight 154 aa, 17 kDa
Observed Molecular Weight 17-20 kDa
GenBank Accession NumberBC012464
Gene Symbol PFDN2
Gene ID (NCBI) 5202
RRIDAB_2877905
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHV9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PFDN2 antibody 13053-1-APDownload protocol
IHC protocol for PFDN2 antibody 13053-1-APDownload protocol
IF protocol for PFDN2 antibody 13053-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols