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PFAS Polyclonal antibody

PFAS Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, Mouse

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 24716-1-AP

Print datasheet

Synonyms

FGAMS, FGARAT, KIAA0361, PFAS, PFAS protein, PURL

验证数据展示

  • WB analysis using 24716-1-AP

    WB analysis using 24716-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24716-1-AP (PFAS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 24716-1-AP

    IF Staining of A431 using 24716-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PFAS antibody (24716-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, Jurkat cells, K-562 cells, NIH/3T3 cells
Positive IF detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24716-1-AP targets PFAS in WB, IF, ELISA applications and shows reactivity with human, Mouse samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity human, Mouse
Immunogen PFAS fusion protein Ag20162 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphoribosylformylglycinamidine synthase
Synonyms FGAMS, FGARAT, KIAA0361, PFAS, PFAS protein, PURL
Calculated Molecular Weight 1338 aa, 145 kDa
Observed Molecular Weight140-150 kDa
GenBank Accession NumberBC167158
Gene Symbol PFAS
Gene ID (NCBI) 5198
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15067
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Phosphoribosylformylglycinamidine synthase (PFAS) is a highly conserved enzyme that catalyzes the fourth step of de novo purine synthesis. It catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.

实验方案

Product Specific Protocols
WB protocol for PFAS antibody 24716-1-APDownload protocol
IF protocol for PFAS antibody 24716-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols