验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
产品信息
20614-1-AP targets PEX7 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag14666 Product name: Recombinant human PEX7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 124-323 aa of BC006268 Sequence: GEQLVVSGSWDQTVKLWDPTVGKSLCTFRGHESIIYSTIWSPHIPGCFASASGDQTLRIWDVKAAGVRIVIPAHQAEILSCDWCKYNENLLVTGAVDCSLRGWDLRNVRQPVFELLGHTYAIRRVKFSPFHASVLASCSYDFTVRFWNFSKPDSLLETVEHHTEFTCGLDFSLQSPTQVADCSWDETIKIYDPACLTIPA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | peroxisomal biogenesis factor 7 |
| 别名 | Peroxin 7, Peroxin-7, PEX, PTS2 receptor, PTS2R |
| 计算分子量 | 323 aa, 36 kDa |
| 观测分子量 | 42 kDa |
| GenBank蛋白编号 | BC006268 |
| 基因名称 | PEX7 |
| Gene ID (NCBI) | 5191 |
| RRID | AB_10694826 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O00628 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PEX7, also known as peroxisomal targeting signal 2 (PTS2) receptor, is one member of peroxins (PEXs) which are proteins required for peroxisome assembly. PEX5 and PEX7 function as receptors that recognize PTS1- and PTS2- containing proteins, respectively. PEX7 plays an essential role in peroxisomal protein import by binding to the N-terminal PTS2-type peroxisomal targeting signal. PEX7 defects are associated with peroxisome biogenesis disorder complementation group 11 (PBD-CG11), rhizomelic chondrodysplasia punctata type 1 (RCDP1) and Refsum disease.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PEX7 antibody 20614-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
PLoS Genet Tysnd1 deficiency in mice interferes with the peroxisomal localization of PTS2 enzymes, causing lipid metabolic abnormalities and male infertility. | ||
PLoS One Pharmacological inhibition of catalase induces peroxisome leakage and suppression of LPS induced inflammatory response in Raw 264.7 cell. |
