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PEX14 Polyclonal antibody

PEX14 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 10594-1-AP

Print datasheet

Synonyms

PTS1 receptor docking protein, Pex14p, PEX, Peroxin-14, Peroxin 14



经过测试的应用

Positive WB detected inA431 cells, HEK-293 cells, HEK-293T cells, HeLa cells, SKOV-3 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inmouse liver tissue, human liver cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, COS7 cells, HepG2 cells, NIH/3T3 cells, C2C12 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10594-1-AP targets PEX14 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, rat, monkey
Cited Reactivityhuman, mouse, rat, chicken, yeast, monkey
Immunogen PEX14 fusion protein Ag0932 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name peroxisomal biogenesis factor 14
Synonyms PTS1 receptor docking protein, Pex14p, PEX, Peroxin-14, Peroxin 14
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC006327
Gene Symbol PEX14
Gene ID (NCBI) 5195
RRIDAB_2252194
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75381
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PEX14 (peroxisomal biogenesis factor 14) is a peroxisomal membrane protein that is essential for protein docking onto the peroxisomes. It is a central component of the peroxisimal matrix protein import machinery and interacts with PEX5 and PEX19. PEX14 is ubiquitously expressed and defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK). This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa (PMID: 16449325; 9653144) and recognize peroxisomal structures in human, monkey and mouse cells.

实验方案

Product Specific Protocols
WB protocol for PEX14 antibody 10594-1-APDownload protocol
IHC protocol for PEX14 antibody 10594-1-APDownload protocol
IF protocol for PEX14 antibody 10594-1-APDownload protocol
IP protocol for PEX14 antibody 10594-1-APDownload protocol
FC protocol for PEX14 antibody 10594-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Science

Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.

Authors - Shun Nagashima
mouseIF

Cell

A Network of Macrophages Supports Mitochondrial Homeostasis in the Heart.

Authors - José A Nicolás-Ávila
humanIF

Mol Cell

The HRI branch of the integrated stress response selectively triggers mitophagy

Authors - Yogaditya Chakrabarty
mouseIF

Mol Cell

Drp1 Tubulates the ER in a GTPase-Independent Manner.

Authors - Yoshihiro Adachi
mouseWB, IHC

Cell Metab

Hif-2α promotes degradation of mammalian peroxisomes by selective autophagy.

Authors - Katharina M Walter
humanWB

Nat Chem Biol

Peroxisomal-derived ether phospholipids link nucleotides to respirasome assembly.

Authors - Christopher F Bennett