验证数据展示

WB analysis using 10594-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10594-1-AP (PEX14 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IHC staining of mouse liver using 10594-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10594-1-AP (PEX14 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 10594-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10594-1-AP (PEX14 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 10594-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PEX14 antibody (10594-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
WB analysis of HEK-293 using 10594-1-AP
WB result of PEX14 antibody (10594-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PEX14 transfected HEK-293 cells.
IP experiment of mouse liver using 10594-1-AP
IP result of anti-PEX14 (IP:10594-1-AP, 3ug; Detection:10594-1-AP 1:500) with mouse liver tissue lysate 4000ug.
FC experiment of HeLa using 10594-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PEX14 (10594-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO验证

PEX14 Polyclonal antibody

PEX14 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10594-1-AP

产品说明书

引用文献(64)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, monkey and More (2)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

别名

NAPP2, Peroxin 14, Peroxin-14, PEX, Pex14p

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

Biotin CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®555 CoraLite®594

规格:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (64)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	A431 cells, HEK-293 cells, HeLa cells
Positive IP detected in	mouse liver tissue
Positive IHC detected in	mouse liver tissue, human liver cancer tissue, human cervical cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, COS7 cells, HepG2 cells, NIH/3T3 cells, C2C12 cells
Positive FC (Intra) detected in	HeLa cells

应用	推荐稀释比
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 2 publications below
WB	See 22 publications below
IHC	See 5 publications below
IF	See 45 publications below

产品信息

10594-1-AP targets PEX14 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

经测试应用	WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用	WB, IHC, IF
经测试反应性	human, mouse, rat, monkey
文献引用反应性	human, mouse, rat, monkey, chicken, yeast
免疫原	PEX14 fusion protein Ag0932 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	peroxisomal biogenesis factor 14
别名	NAPP2, Peroxin 14, Peroxin-14, PEX, Pex14p
计算分子量	41 kDa
观测分子量	57 kDa
GenBank蛋白编号	BC006327
基因名称	PEX14
Gene ID (NCBI)	5195
RRID	AB_2252194
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	O75381
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

PEX14 (peroxisomal biogenesis factor 14) is a peroxisomal membrane protein that is essential for protein docking onto the peroxisomes. It is a central component of the peroxisimal matrix protein import machinery and interacts with PEX5 and PEX19. PEX14 is ubiquitously expressed and defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK). This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa (PMID: 16449325; 9653144) and recognize peroxisomal structures in human, monkey and mouse cells.

实验方案

Product Specific Protocols
WB protocol for PEX14 antibody 10594-1-AP	Download protocol
IHC protocol for PEX14 antibody 10594-1-AP	Download protocol
IF protocol for PEX14 antibody 10594-1-AP	Download protocol
IP protocol for PEX14 antibody 10594-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human		Science Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division. Authors - Shun Nagashima View Article
mouse	IF	Cell A Network of Macrophages Supports Mitochondrial Homeostasis in the Heart. Authors - José A Nicolás-Ávila View Article
mouse	IF	Nat Cell Biol Blebbisomes are large, organelle-rich extracellular vesicles with cell-like properties Authors - Dennis K Jeppesen View Article
human	IF	Mol Cell The HRI branch of the integrated stress response selectively triggers mitophagy Authors - Yogaditya Chakrabarty View Article
mouse	IF	Mol Cell Drp1 Tubulates the ER in a GTPase-Independent Manner. Authors - Yoshihiro Adachi View Article
mouse	WB,IHC	Cell Metab Hif-2α promotes degradation of mammalian peroxisomes by selective autophagy. Authors - Katharina M Walter View Article

验证数据展示

WB analysis using 10594-1-AP

IHC staining of mouse liver using 10594-1-AP

IHC staining of mouse liver using 10594-1-AP

IF Staining of HeLa using 10594-1-AP

WB analysis of HEK-293 using 10594-1-AP

IP experiment of mouse liver using 10594-1-AP

FC experiment of HeLa using 10594-1-AP

PEX14 Polyclonal antibody

PEX14 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.

A Network of Macrophages Supports Mitochondrial Homeostasis in the Heart.

Blebbisomes are large, organelle-rich extracellular vesicles with cell-like properties

The HRI branch of the integrated stress response selectively triggers mitophagy

Drp1 Tubulates the ER in a GTPase-Independent Manner.

Hif-2α promotes degradation of mammalian peroxisomes by selective autophagy.