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- KD/KO Validated
PEX14 Polyclonal antibody
PEX14 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, monkey and More (1)
Applications
FC, IF, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, HEK-293 cells, HEK-293T cells, HeLa cells, SKOV-3 cells |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | human liver cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells, COS7 cells, HepG2 cells, NIH/3T3 cells, C2C12 cells |
Positive FC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:200 |
Immunofluorescence (IF) | IF : 1:500-1:2000 |
Sample-dependent, check data in validation data gallery |
产品信息
10594-1-AP targets PEX14 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
Tested Applications | FC, IF, IHC, IP, WB, ELISA |
Cited Applications | IF, IHC, WB |
Tested Reactivity | human, mouse, rat, monkey |
Cited Reactivity | human, mouse, rat, yeast, monkey |
Immunogen | PEX14 fusion protein Ag0932 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | peroxisomal biogenesis factor 14 |
Synonyms | Cell and organelle markers, NAPP2, Peroxin 14, Peroxisome Marker, PEX14, Pex14p, PTS1 receptor docking protein |
Calculated molecular weight | 41 kDa |
Observed molecular weight | 57 kDa |
GenBank accession number | BC006327 |
Gene symbol | PEX14 |
Uniprot | O75381 |
Gene ID (NCBI) | 5195 |
RRID | AB_2252194 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for PEX14 antibody 10594-1-AP | Download protocol |
IHC protocol for PEX14 antibody 10594-1-AP | Download protocol |
IF protocol for PEX14 antibody 10594-1-AP | Download protocol |
IP protocol for PEX14 antibody 10594-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Science Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division. | ||
Nat Chem Biol Peroxisomal-derived ether phospholipids link nucleotides to respirasome assembly. | ||
Nat Commun Limited survival and impaired hepatic fasting metabolism in mice with constitutive Rag GTPase signaling. |