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PEX13 Polyclonal antibody

PEX13 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 26649-1-AP

Print datasheet

Synonyms

NALD, Peroxin 13, PEX13, ZWS

验证数据展示

  • WB analysis of Jurkat using 26649-1-AP

    WB analysis of Jurkat using 26649-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26649-1-AP (PEX13 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 26649-1-AP

    IHC staining of human kidney using 26649-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26649-1-AP (PEX13 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 26649-1-AP

    IHC staining of human kidney using 26649-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26649-1-AP (PEX13 antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 26649-1-AP

    IF Staining of HeLa using 26649-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 26649-1-AP (PEX13 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells, HeLa cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

The immunogen of 26649-1-AP is PEX13 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen PEX13 fusion protein Ag24400 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name peroxisomal biogenesis factor 13
Synonyms NALD, Peroxin 13, PEX13, ZWS
Calculated Molecular Weight 403 aa, 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC067090
Gene Symbol PEX13
Gene ID (NCBI) 5194
RRIDAB_2880588
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92968
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PEX13 antibody 26649-1-APDownload protocol
IHC protocol for PEX13 antibody 26649-1-APDownload protocol
IF protocol for PEX13 antibody 26649-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Autophagy

Silencing PEX26 as an unconventional mode to kill drug-resistant cancer cells and forestall drug resistance.

Authors - Michael S Dahabieh
View Article
humanWB

Biol Chem

Comparison of human PEX knockout cell lines suggests a dual role of PEX1 in peroxisome biogenesis

Authors - Julia Ott
View Article