验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells, K-562 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
产品信息
26649-1-AP targets PEX13 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human, pig |
| 免疫原 |
CatNo: Ag24400 Product name: Recombinant human PEX13 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 253-403 aa of BC067090 Sequence: LLSTHSDEVTDSINWASGEDDHVVARAEYDFAAVSEEEISFRAGDMLNLALKEQQPKVRGWLLASLDGQTTGLIPANYVKILGKRKGRKTVESSKVSKQQQSFTNPTLTKGATVADSLDEQEAAFESVFVETNKVPVAPDSIGKDGEKQDL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | peroxisomal biogenesis factor 13 |
| 别名 | Peroxin-13, Peroxisomal membrane protein PEX13, PEX |
| 计算分子量 | 403 aa, 44 kDa |
| 观测分子量 | 44 kDa |
| GenBank蛋白编号 | BC067090 |
| 基因名称 | PEX13 |
| Gene ID (NCBI) | 5194 |
| RRID | AB_2880588 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92968 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for PEX13 antibody 26649-1-AP | Download protocol |
| IHC protocol for PEX13 antibody 26649-1-AP | Download protocol |
| WB protocol for PEX13 antibody 26649-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Autophagy Silencing PEX26 as an unconventional mode to kill drug-resistant cancer cells and forestall drug resistance. | ||
Biol Chem Comparison of human PEX knockout cell lines suggests a dual role of PEX1 in peroxisome biogenesis | ||
Nat Commun Modulation of peroxisomal import by the PEX13 SH3 domain and a proximal FxxxF binding motif | ||
mBio PEDV NSP8 inhibits IFN-III production induced by MAVS through downregulation of PEX13.
| ||
Nat Commun A mitochondria-driven quality control mechanism for peroxisomal membrane proteins. | ||
J Virol Cyclo-C stabilizes PEX13 to inhibit porcine epidemic diarrhea virus replication by blocking pexophagy-mediated disruption of antiviral innate immunity. |
