验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, MDA-MB-231 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
27502-1-AP targets PEX10 in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag26665 Product name: Recombinant human PEX10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 111-160 aa of BC018198 Sequence: LPLEQELQADPDSGRPLQGSLGPGGRGCSGARRWMRHHTATLTEQQRRAL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | peroxisomal biogenesis factor 10 |
| 别名 | NALD, Peroxin 10, Peroxisome assembly protein 10, PEX10, RING finger protein 69, RNF69 |
| 计算分子量 | 37 kDa |
| 观测分子量 | 37-50 kDa |
| GenBank蛋白编号 | BC018198 |
| 基因名称 | PEX10 |
| Gene ID (NCBI) | 5192 |
| RRID | AB_2918125 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O60683 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Peroxisome biogenesis factor 10 (PEX10) is involved in the import of peroxisomal matrix proteins, and the mutation of this gene causes 3 subtypes of peroxisome biogenesis disorders, namely Zellweger syndrome (severe), neonatal adrenoleukodystrophy (moderate) and an ataxic form (mild). ( PMID: 28320181)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PEX10 antibody 27502-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
