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验证数据展示

  • WB analysis using 88220-2-RR (same clone as 88220-2-PBS)

    WB analysis using 88220-2-RR (same clone as 88220-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88220-2-RR (PERK/EIF2AK3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88220-2-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 88220-2-RR (same clone as 88220-2-PBS)

    IP experiment of HeLa using 88220-2-RR (same clone as 88220-2-PBS)

    IP result of anti-PERK/EIF2AK3 (IP:88220-2-RR, 4ug; Detection:88220-2-RR 1:800) with HeLa cells lysate 1200 ug. This data was developed using the same antibody clone with 88220-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 88220-2-RR (same clone as 88220-2-PBS)

    FC experiment of HeLa using 88220-2-RR (same clone as 88220-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PERK/EIF2AK3 Recombinant monoclonal antibody (88220-2-RR, Clone:260327C7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 88220-2-PBS in a different storage buffer formulation.

PERK/EIF2AK3 Recombinant monoclonal antibody, PBS Only

PERK/EIF2AK3 Uni-rAb® Recombinant Antibody for WB, FC (Intra), IP, Indirect ELISA
Cat No. 88220-2-PBS

产品说明书

CloneNo. 260327C7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), IP, Indirect ELISA

别名

EIF2AK3, PERK, EC:2.7.11.1, EIF2AK 3, Eukaryotic translation initiation factor 2-alpha kinase 3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

88220-2-PBS targets PERK/EIF2AK3 in WB, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag18254

Product name: Recombinant human EIF2AK3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 767-1116 aa of BC126354

Sequence: TDCDVEDGTMDGNDEGHSFELCPSEASPYVRSRERTSSSIVFEDSGCDNASSKEEPKTNRLHIGNHCANKLTAFKPTSSKSSSEATLSISPPRPTTLSLDLTKNTTEKLQPSSPKVYLYIQMQLCRKENLKDWMNGRCTIEERERSVCLHIFLQIAEAVEFLHSKGLMHRDLKPSNIFFTMDDVVKVGDFGLVTAMDQDEEEQTVLTPMPAYARHTGQVGTKLYMSPEQIHGNSYSHKVDIFSLGLILFELLYPFSTQMERVRTLTDVRNLKFPPLFTQKYPCEYVMVQDMLSPSPMERPEAINIIENAVFEDLDFPGKTVLRQRSRSLSSSGTKHSRQSNNSHSPLPSN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 eukaryotic translation initiation factor 2-alpha kinase 3
别名 EIF2AK3, PERK, EC:2.7.11.1, EIF2AK 3, Eukaryotic translation initiation factor 2-alpha kinase 3
计算分子量 1116 aa, 125 kDa
观测分子量 140 kDa
GenBank蛋白编号BC126354
基因名称 PERK
Gene ID (NCBI) 9451
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NZJ5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PERK, also named as EIF2AK3 or PEK, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and GCN2 subfamily. It acts potentially as a metabolic sensor in the INS-secreting beta-cells to modulate the trafficking and quality control of pro-INS in the ER relative to the physiological demands for circulating INS (PMID:20530744). PERK and EIF2AK4 also have a functional role on regulating translation under non-stressed conditions, apart from their long established roles as stress kinases (PMID: 21638082). Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS). The molecular mass of PERK is 125 kDa and can be detected as 140 kDa or 170 kDa due to some modifications such as glycosylation and phosphorylation (PMID: 20851775, 24371088, 21703327).

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