PER3 Polyclonal antibody
PER3 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
产品信息
12550-1-AP targets PER3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, WB,ELISA |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | PER3 fusion protein Ag3400 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | period homolog 3 (Drosophila) |
| Synonyms | GIG13, hPER3, PER3, period homolog 3 (Drosophila) |
| Calculated Molecular Weight | 1201 aa, 132 kDa |
| Observed Molecular Weight | 132 kDa |
| GenBank Accession Number | BC026102 |
| Gene Symbol | PER3 |
| Gene ID (NCBI) | 8863 |
| RRID | AB_2161814 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P56645 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The suprachiasmatic nuclei (SCN) of the anterior hypothalamus function as a circadian clock in the mammalian brain, generating circadian rhythms in physiology and behavior. The period homolog genesPer3 is an important component of the circadian clock system. PER3 plays a role in cell proliferation, apoptosis,DNA damage response, cell cycle control, and treatment response of chemotherapy agents in cancers.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PER3 antibody 12550-1-AP | Download protocol |
| IHC protocol for PER3 antibody 12550-1-AP | Download protocol |
| IF protocol for PER3 antibody 12550-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Arthritis Res Ther Dark-light cycle disrupts bone metabolism and suppresses joint deterioration in osteoarthritic rats. | ||
Int J Biochem Cell Biol TFEB Regulates PER3 Expression via Glucose-dependent Effects on CLOCK/BMAL1. | ||
Biochem Biophys Res Commun Loss of circadian protein TIMELESS accelerates the progression of cellular senescence. | ||
Adv Sci (Weinh) The PRMT6/PARP1/CRL4B Complex Regulates the Circadian Clock and Promotes Breast Tumorigenesis | ||
Anesth Analg Sleep Fragmentation, Electroencephalographic Slowing, and Circadian Disarray in a Mouse Model for Intensive Care Unit Delirium |