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验证数据展示

  • WB analysis using 67513-1-Ig (same clone as 67513-1-PBS)

    WB analysis using 67513-1-Ig (same clone as 67513-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67513-1-Ig (PER2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67513-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67513-1-Ig (same clone as 67513-1-PBS)

    IF Staining of HeLa using 67513-1-Ig (same clone as 67513-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PER2 (isoform 1) antibody (67513-1-Ig, Clone: 2E6C3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67513-1-PBS in a different storage buffer formulation.

PER2 (isoform 1) Monoclonal antibody, PBS Only

PER2 (isoform 1) Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67513-1-PBS

产品说明书

CloneNo. 2E6C3

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

PER2, PER 2, hPER2, FASPS, 2E6C3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PER2 Ready-To-Use IHC Kit. PER2 primary antibody included.

产品信息

67513-1-PBS targets PER2 (isoform 1) in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag29489

Product name: Recombinant human PER2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 740-839 aa of NM_022817

Sequence: SFLQKFKEIRKLSIFQSHCHYYLQERSKGQPSERTAPGLRNTSGIDSPWKKTGKNRKLKSKRVKPRDSSESTGSGGPVSARPPLVGLNATAWSPSDTSQS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 period homolog 2 (Drosophila)
别名 PER2, PER 2, hPER2, FASPS, 2E6C3
计算分子量 137 kDa
观测分子量150~160 kDa
GenBank蛋白编号NM_022817
基因名称 PER2
Gene ID (NCBI) 8864
RRIDAB_2882734
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO15055
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PER2, also named as KIAA0347, is a component of the circadian clock mechanism which is essential for generating circadian rhythms. Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) which is characterized by very early sleep onset and offset. There are two isoforms of PER2: isoform 1 has an expected molecular size of about 140 kDa, and 67513-1-Ig detected molecular weight of 150-160 kDa (PMID:16675517), while isoform 2, known as PER2S (a second splicing variant of the human PER2 gene), has a molecular weight of about 45 kDa (PMID:26347774).

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