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PER2 Polyclonal antibody

PER2 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 29954-1-AP

Print datasheet

Synonyms

FASPS, hPER2, KIAA0347, PER2, period homolog 2 (Drosophila)

验证数据展示

  • WB analysis using 29954-1-AP

    WB analysis using 29954-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29954-1-AP (PER2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of MDA-MB-231 using 29954-1-AP

    IF Staining of MDA-MB-231 using 29954-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MDA-MB-231 cells using PER2 antibody (29954-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inBxPC-3 cells, HeLa cells
Positive IF detected inMDA-MB-231 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29954-1-AP targets PER2 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human
Immunogen PER2 fusion protein Ag31471 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name period homolog 2 (Drosophila)
Synonyms FASPS, hPER2, KIAA0347, PER2, period homolog 2 (Drosophila)
Calculated Molecular Weight137KD
Observed Molecular Weight45 kDa
GenBank Accession NumberNM_022817
Gene Symbol PER2
Gene ID (NCBI) 8864
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15055
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PER2, also named as KIAA0347, is a component of the circadian clock mechanism which is essential for generating circadian rhythms. Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) which is characterized by very early sleep onset and offset. There are two isoforms of PER2: isoform 1 has an expected molecular size of about 140 kDa, while isoform 2, known as PER2S (a second splicing variant of the human PER2 gene), has a molecular weight of about 45 kDa (PMID:26347774). 29954-1-AP detected molecular weight of 45 kDa.

实验方案

Product Specific Protocols
WB protocol for PER2 antibody 29954-1-APDownload protocol
IF protocol for PER2 antibody 29954-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols