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验证数据展示

  • WB analysis using 29954-1-AP

    WB analysis using 29954-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29954-1-AP (PER2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of MDA-MB-231 using 29954-1-AP

    IF Staining of MDA-MB-231 using 29954-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MDA-MB-231 cells using PER2 antibody (29954-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

PER2 Polyclonal antibody

PER2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 29954-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

FASPS, hPER2, KIAA0347, PER2, period homolog 2 (Drosophila)

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inBxPC-3 cells, HeLa cells
Positive IF/ICC detected inMDA-MB-231 cells
Planning an IHC experiment? We recommend our IHCeasy PER2 Ready-To-Use IHC Kit. PER2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29954-1-AP targets PER2 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 PER2 fusion protein Ag31471 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 period homolog 2 (Drosophila)
别名 FASPS, hPER2, KIAA0347, PER2, period homolog 2 (Drosophila)
计算分子量137KD
观测分子量45 kDa
GenBank蛋白编号NM_022817
基因名称 PER2
Gene ID (NCBI) 8864
RRIDAB_3086198
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15055
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PER2, also named as KIAA0347, is a component of the circadian clock mechanism which is essential for generating circadian rhythms. Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) which is characterized by very early sleep onset and offset. There are two isoforms of PER2: isoform 1 has an expected molecular size of about 140 kDa, while isoform 2, known as PER2S (a second splicing variant of the human PER2 gene), has a molecular weight of about 45 kDa (PMID:26347774). 29954-1-AP detected molecular weight of 45 kDa.

实验方案

Product Specific Protocols
WB protocol for PER2 antibody 29954-1-APDownload protocol
IF protocol for PER2 antibody 29954-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols