验证数据展示
经过测试的应用
| Positive WB detected in | BxPC-3 cells, Y79 cells, K-562 cells, L02 cells |
| Positive IP detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20359-1-AP targets PER2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IP, IF, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, goat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | period homolog 2 (Drosophila) |
| 别名 | FASPS, hPER2, KIAA0347, PER2, period homolog 2 (Drosophila) |
| 计算分子量 | 137 kDa |
| 观测分子量 | 136 kDa |
| GenBank蛋白编号 | NM_022817 |
| 基因名称 | PER2 |
| Gene ID (NCBI) | 8864 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O15055 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PER2, also named as KIAA0347, is a component of the circadian clock mechanism which is essential for generating circadian rhythms. PER2 is a negative element in the circadian transcriptional loop. PER2 influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. It negatively regulates CLOCK|NPAS2-BMAL1|BMAL2-induced transactivation. Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) which is characterized by very early sleep onset and offset. The antibody is specific to PER2. It can recognize isoform1 and isoform2 of PER2.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for PER2 antibody 20359-1-AP | Download protocol |
| WB protocol for PER2 antibody 20359-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv Time-of-day specificity of anticancer drugs may be mediated by circadian regulation of the cell cycle. | ||
Nat Commun Correspondence: Oncogenic MYC persistently upregulates the molecular clock component REV-ERBα. | ||
Elife NRF2 regulates core and stabilizing circadian clock loops, coupling redox and timekeeping inMus musculus. |
