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验证数据展示

  • WB analysis using 13463-1-AP (same clone as 13463-1-PBS)

    WB analysis using 13463-1-AP (same clone as 13463-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13463-1-AP (PER1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13463-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 13463-1-AP (same clone as 13463-1-PBS)

    IF Staining of HeLa using 13463-1-AP (same clone as 13463-1-PBS)

    Immunofluorescent analysis of Hela cells, using PER1 antibody 13463-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 13463-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 13463-1-AP (same clone as 13463-1-PBS)

    IHC staining of human breast cancer using 13463-1-AP (same clone as 13463-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13463-1-AP (PER1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13463-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 13463-1-AP (same clone as 13463-1-PBS)

    IHC staining of human breast cancer using 13463-1-AP (same clone as 13463-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13463-1-AP (PER1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13463-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 13463-1-AP (same clone as 13463-1-PBS)

    IHC staining of human tonsillitis using 13463-1-AP (same clone as 13463-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13463-1-AP (PER1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13463-1-PBS in a different storage buffer formulation.

PER1 Polyclonal antibody, PBS Only

PER1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13463-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

hPER, hPER1, KIAA0482, PER, period homolog 1 (Drosophila)

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

13463-1-PBS targets PER1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag4249

Product name: Recombinant human PER1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-346 aa of BC028207

Sequence: MSGPLEGADGGGDPRPGESFCPGGVPSPGPPQHRPCPGPSLADDTDANSNGSSGNESNGHESRGASQRSSHSSSSGNGKDSALLETTESSKSTNSQSPSPPSSSIAYSLLSASSEQDNPSTSGCSSEQSARARTQKELMTALRELKLRLPPERRGKGRSGTLATLQYALACVKQVQANQEYYQQWSLEEGEPCSMDMSTYTLEELEHITSEYTLQNQDTFSVAVSFLTGRIVYISEQAAVLLRCKRDVFRGTRFSELLAPQDVGVFYGSTAPSRLPTWGTGASAGSGLRDFTQEKSVFCRIRGGPDRDPGPRYQPFRLTPYVTKIRVSDGAPAQPCCLLIAE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 period homolog 1 (Drosophila)
别名 hPER, hPER1, KIAA0482, PER, period homolog 1 (Drosophila)
计算分子量 1290 aa, 136 kDa
观测分子量 110 kDa, 50-55 kDa
GenBank蛋白编号BC028207
基因名称 PER1
Gene ID (NCBI) 5187
RRIDAB_2161536
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15534
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PER1 is a master regulator of circadian rhythm and functions in the nucleus to repress expression of the central circadian clock genes. The periodicity of PER1 abundance, nuclear translocation, and transcriptional repression is regulated by PER1 phosphorylation, ubiquitination, and proteasomal degradation [PMID: 21514639]. It is one component of the circadian clock mechanism which is essential for generating circadian rhythms. It can influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus [PMID: 9856465, 20930143]. In addition to bands of the predicted molecular mass of 140 kDa, additional PER1 species of 55 and 45 kDa is detected that is predominantly nuclear [PMID: 11726537] .

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