验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, HEK-293 cells, human placenta tissue, NCCIT cells, HeLa cells, Jurkat cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
67202-1-Ig targets PEPD in WB, IHC, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag28713 Product name: Recombinant human PEPD protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 192-439 aa of BC015027 Sequence: LEVLRYTNKISSEAHREVMKAVKVGMKEYELESLFEHYCYSRGGMRHSSYTCICGSGENSAVLHYGHAGAPNDRTIQNGDMCLFDMGGEYYCFASDITCSFPANGKFTADQKAVYEAVLRSSRAVMGAMKPGVWWPDMHRLADRIHLEELAHMGILSGSVDAMVQAHLGAVFMPHGLGHFLGIDVHDVGGYPEGVERIDEPGLRSLRTARHLQPGMVLTVEPGIYFIDHLLDEALADPARASFLNREV 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | peptidase D |
| 别名 | Imidodipeptidase, PEPD, peptidase D, PRD, PROLIDASE, Proline dipeptidase, X Pro dipeptidase, Xaa Pro dipeptidase |
| 计算分子量 | 493 aa, 55 kDa |
| 观测分子量 | 55 kDa |
| GenBank蛋白编号 | BC015027 |
| 基因名称 | PEPD |
| Gene ID (NCBI) | 5184 |
| RRID | AB_2882495 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P12955 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PEPD, also named as PRD, Prolidase, X-Pro dipeptidase, Imidodipeptidase, Peptidase D and Proline dipeptidase, belongs to the peptidase M24B family and Eukaryotic-type prolidase subfamily. PEPD splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. It plays an important role in collagen metabolism because the high level of iminoacids in collagen. Defects in PEPD are a cause of prolidase deficiency (PD). PEPD is considered as the most promising candidate genes for altering AAA risk, based on gene function, association evidence, gene expression, and protein expression.(PMID:21247474)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PEPD antibody 67202-1-Ig | Download protocol |
| IHC protocol for PEPD antibody 67202-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
