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验证数据展示

  • WB analysis of SH-SY5Y using 85099-1-RR (same clone as 85099-1-PBS)

    WB analysis of SH-SY5Y using 85099-1-RR (same clone as 85099-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 85099-1-RR (PEMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85099-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 85099-1-RR (same clone as 85099-1-PBS)

    IHC staining of mouse liver using 85099-1-RR (same clone as 85099-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85099-1-RR (PEMT antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85099-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 85099-1-RR (same clone as 85099-1-PBS)

    IHC staining of mouse liver using 85099-1-RR (same clone as 85099-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85099-1-RR (PEMT antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85099-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85099-1-RR

    Affinity and Kinetic Characterization of 85099-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85099-1-RR against Human PEMT were performed. The affinity constant is 0.379 nM.

PEMT Recombinant antibody, PBS Only

PEMT Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85099-1-PBS

产品说明书

CloneNo. 242150D5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

Phosphatidylethanolamine N-methyltransferase, PEMT2, PEMPT, PEAMT, EC:2.1.1.17

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85099-1-PBS targets PEMT in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 PEMT fusion protein Ag8034 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 phosphatidylethanolamine N-methyltransferase
别名 Phosphatidylethanolamine N-methyltransferase, PEMT2, PEMPT, PEAMT, EC:2.1.1.17
计算分子量 26 kDa
观测分子量 22-25 kDa
GenBank蛋白编号BC050593
基因名称 PEMT
Gene ID (NCBI) 10400
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UBM1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PEMT Catalyzes the three sequential steps of the methylation pathway for the biosynthesis of phosphatidylcholine, a critical and essential component for membrane structure (PMID:12431977, 15927961).