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PEMT Polyclonal antibody

PEMT Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 25916-1-AP

Print datasheet

Synonyms

PEAMT, PEMPT, PEMT, PEMT2, PNMT

验证数据展示

  • WB analysis of rat liver using 25916-1-AP

    WB analysis of rat liver using 25916-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 25916-1-AP (PEMT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 25916-1-AP

    IHC staining of human liver using 25916-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25916-1-AP (PEMT Antibody) at dilution of 1:400 (under 40x lens).

经过测试的应用

Positive WB detected inrat liver tissue, mouse liver tissue
Positive IHC detected inhuman liver tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25916-1-AP targets PEMT in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, rat, mouse
Cited Reactivityhuman
Immunogen PEMT fusion protein Ag5872 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphatidylethanolamine N-methyltransferase
Synonyms PEAMT, PEMPT, PEMT, PEMT2, PNMT
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 22-25 kDa
GenBank Accession NumberBC050593
Gene Symbol PEMT
Gene ID (NCBI) 10400
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBM1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PEMT antibody 25916-1-APDownload protocol
IHC protocol for PEMT antibody 25916-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Cell Metab

Transsulfuration Activity Can Support Cell Growth upon Extracellular Cysteine Limitation.

Authors - Jiajun Zhu
View Article