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PELP1 Monoclonal antibody

PELP1 Monoclonal Antibody for FC (Intra), IF-P, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (1)

Applications

FC (Intra), IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B11E4

Cat No : 67050-1-Ig

Print datasheet

Synonyms

HMX3, MNAR, P160, PELP1, Transcription factor HMX3

验证数据展示

  • WB analysis using 67050-1-Ig

    WB analysis using 67050-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67050-1-Ig (PELP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 67050-1-Ig

    IHC staining of human cervical cancer using 67050-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67050-1-Ig (PELP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PELP1 antibody included
  • IHC staining of human cervical cancer using 67050-1-Ig

    IHC staining of human cervical cancer using 67050-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67050-1-Ig (PELP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PELP1 antibody included
  • IF Staining of human breast cancer using 67050-1-Ig

    IF Staining of human breast cancer using 67050-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using PELP1 antibody (67050-1-Ig, Clone: 1B11E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of MCF-7 using 67050-1-Ig

    FC experiment of MCF-7 using 67050-1-Ig

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human PELP1 (67050-1-Ig, Clone:1B11E4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of human breast cancer using 67050-1-Ig

    IF Staining of human breast cancer using 67050-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using PELP1 antibody (67050-1-Ig, Clone: 1B11E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, T-47D cells, Jurkat cells, HEK-293 cells, MCF-7 cells
Positive IHC detected inhuman cervical cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue
Positive FC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy PELP1 Ready-To-Use IHC Kit. PELP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67050-1-Ig targets PELP1 in WB, IF, IHC, ELISA, FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra), IF-P, IHC, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity Human
Cited Reactivitymouse
Immunogen PELP1 fusion protein Ag25729 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name proline, glutamate and leucine rich protein 1
Synonyms HMX3, MNAR, P160, PELP1, Transcription factor HMX3
Calculated Molecular Weight 1130 aa, 120 kDa
Observed Molecular Weight 160 kDa
GenBank Accession NumberBC069058
Gene Symbol PELP1
Gene ID (NCBI) 27043
RRIDAB_2882363
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8IZL8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PELP1 was first identified as a 160 kDa protein in a screen for Src homology 2 (SH2) domain-binding proteins. PELP1 is overexpressed in 60-80% of breast tumors and plays important roles in both ER genomic and non-genomic signaling. In vivo, PELP1 subcellular localization is primarily nuclear in normal breast tissue, but it is localized to the cytoplasm in about 40% of invasive breast tumors. In the nucleus, PELP1 interacts with a number of transcription factors. The proto-oncogenic functions of PELP1 involve different cellular processes including epigenetic modifications leading to ER transactivation and breast cancer progression. Furthermore, PELP1 activates kinase cascades in the cytoplasm such as MAPK activation via c-Src and PI3K signaling.

实验方案

Product Specific Protocols
WB protocol for PELP1 antibody 67050-1-IgDownload protocol
IHC protocol for PELP1 antibody 67050-1-IgDownload protocol
IF protocol for PELP1 antibody 67050-1-IgDownload protocol
FC protocol for PELP1 antibody 67050-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC

Int Immunopharmacol

MiR-195-5p is involved in testicular ischemia/reperfusion injury by directly targeting PELP1 and regulating spermatogonia pyroptosis

Authors - Kai-Xiang He
View Article