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验证数据展示

  • WB analysis using 30135-1-AP (same clone as 30135-1-PBS)

    WB analysis using 30135-1-AP (same clone as 30135-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30135-1-AP (PELP1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30135-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 30135-1-AP (same clone as 30135-1-PBS)

    IF Staining of MCF-7 using 30135-1-AP (same clone as 30135-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PELP1 antibody (30135-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30135-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 30135-1-AP (same clone as 30135-1-PBS)

    IHC staining of mouse testis using 30135-1-AP (same clone as 30135-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30135-1-AP (PELP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30135-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PELP1 antibody included
  • IHC staining of mouse testis using 30135-1-AP (same clone as 30135-1-PBS)

    IHC staining of mouse testis using 30135-1-AP (same clone as 30135-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30135-1-AP (PELP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30135-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PELP1 antibody included

PELP1 Polyclonal antibody, PBS Only

PELP1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 30135-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PELP1 Ready-To-Use IHC Kit. PELP1 primary antibody included.

产品信息

30135-1-PBS targets PELP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag32720

Product name: Recombinant human PELP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1000-1130 aa of BC069058

Sequence: EPEPGLLLEVEEPGTEEERGADTAPTLAPEALPSQGEVEREGESPAAGPPPQELVEEEPSAPPTLLEEETEDGSDKVQPPPETPAEEEMETETEAEALQEKEQDDTAAMLADFIDCPPDDEKPPPPTEPDS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proline, glutamate and leucine rich protein 1
别名
计算分子量 1130 aa, 120 kDa
观测分子量 160 kDa
GenBank蛋白编号BC069058
基因名称 PELP1
Gene ID (NCBI) 27043
RRIDAB_2935519
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IZL8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PELP1 was first identified as a 160 kDa protein in a screen for Src homology 2 (SH2) domain-binding proteins. PELP1 is overexpressed in 60-80% of breast tumors and plays important roles in both ER genomic and non-genomic signaling. In vivo, PELP1 subcellular localization is primarily nuclear in normal breast tissue, but it is localized to the cytoplasm in about 40% of invasive breast tumors. In the nucleus, PELP1 interacts with a number of transcription factors. The proto-oncogenic functions of PELP1 involve different cellular processes including epigenetic modifications leading to ER transactivation and breast cancer progression. Furthermore, PELP1 activates kinase cascades in the cytoplasm such as MAPK activation via c-Src and PI3K signaling.

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