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验证数据展示

  • IHC staining of mouse brain using 21446-1-AP

    IHC staining of mouse brain using 21446-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21446-1-AP (PEA15 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 21446-1-AP

    IHC staining of mouse brain using 21446-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21446-1-AP (PEA15 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of fetal human brain using 21446-1-AP

    WB analysis of fetal human brain using 21446-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 21446-1-AP (PEA15 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

PEA15 Polyclonal antibody

PEA15 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21446-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

HMAT1, HUMMAT1H, MAT1, MAT1H, PEA 15, PEA15, PED

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected infetal human brain tissue
Positive IHC detected inmouse brain tissue, human heart tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21446-1-AP targets PEA15 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag13782

Product name: Recombinant human PEA15 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-130 aa of BC002426

Sequence: MAEYGTLLQDLTNNITLEDLEQLKSACKEDIPSEKSEEITTGSAWFSFLESHNKLDKDNLSYIEHIFEISRRPDLLTMVVDYRTRVLKISEEDELDTKLTRIPSAKKYKDIIRQPSEEEIIKLAPPPKKA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphoprotein enriched in astrocytes 15
别名 HMAT1, HUMMAT1H, MAT1, MAT1H, PEA 15, PEA15, PED
计算分子量 130 aa, 15 kDa
观测分子量 15-18 kDa
GenBank蛋白编号BC002426
基因名称 PEA15
Gene ID (NCBI) 8682
RRIDAB_2878858
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15121
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PEA15 antibody 21446-1-APDownload protocol
IHC protocol for PEA15 antibody 21446-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

J Cancer

The microRNA212 regulated PEA15 promotes ovarian cancer progression by inhibiting of apoptosis.

Authors - Yonghong Luo
  • KD Validated
View Article