验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, HEK-293 cells |
| Positive IP detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10507-2-AP targets PDZK1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag0787 Product name: Recombinant human PDZK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC006496 Sequence: MTSTFNPRECKLSKQEGQNYGFFLRIEKDTEGHLVRVVEKCSPAEKAGLQDGDRVLRINGVFVDKEEHMQVVDLVRKSGNSVTLLVLDGDSYEKAVKTRVDLKELGQSQKEQGLSDNILSPVMNGGVQTWTQPRLCYLVKEGGSYGFSLKTVQGKKGVYMTDITPQGVAMRAGVLADDHLIEVNGENVEDASHEEVVEKVKKSGSRVMFLLVDKETDKRHVEQKIQFKRETASLKLLPHQPRIVEMKKGSNGYGFYLRAGSEQKGQIIKDIDSGSPAEEAGLKNNDLVVAVNGESVETLD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | PDZ domain containing 1 |
| 别名 | CAP70, CFTR-associated protein of 70 kDa, Na(+)/H(+) exchange regulatory cofactor NHE-RF3, Na/Pi cotransporter C-terminal-associated protein 1, NaPi-Cap1 |
| 计算分子量 | 57 kDa |
| 观测分子量 | 63-70 kDa |
| GenBank蛋白编号 | BC006496 |
| 基因名称 | PDZK1 |
| Gene ID (NCBI) | 5174 |
| RRID | AB_2299495 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5T2W1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDZK1 is a PDZ domain-containing membrane scaffold protein widely expressed in a variety of epithelial cells. It plays a key role in cell signal transduction and ion channel regulation by interacting with a variety of ion channels, transporters, and receptors. PDZK1 simultaneously anchors multiple membrane proteins through its PDZ domain to regulate membrane expression and activity. PDZK1 maintains cholesterol homeostasis and participates in the regulation of the urate/anion exchanger (URAT1) in renal tubular uric acid reabsorption.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PDZK1 antibody 10507-2-AP | Download protocol |
| IP protocol for PDZK1 antibody 10507-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncotarget Downregulation of vimentin expression increased drug resistance in ovarian cancer cells. | ||
Mol Med PDZK1 Is a Novel Factor in Breast Cancer That Is Indirectly Regulated by Estrogen through IGF-1R and Promotes Estrogen-Mediated Growth. | ||
Mol Med Correlation between PDZK1, Cdc37, Akt and breast cancer malignancy: the role of PDZK1 in cell growth through Akt stabilization by increasing and interacting with Cdc37. | ||
Cell Div miR-101-3p-mediated role of PDZK1 in hepatocellular carcinoma progression and the underlying PI3K/Akt signaling mechanism
| ||
Aging (Albany NY) Promoter hypomethylated PDZK1 acts as a tumorigenic gene in glioma by interacting with AKT1
| ||
Bone Res PDZK1 protects against mechanical overload-induced chondrocyte senescence and osteoarthritis by targeting mitochondrial function |
