验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, HEK-293 cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 2 publications below |
产品信息
25512-1-AP targets PDZD8 in WB, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | PDZD8 fusion protein Ag21914 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | PDZ domain containing 8 |
| 别名 | PDZ domain containing 8, PDZD8, PDZK8 |
| 计算分子量 | 1154 aa, 129 kDa |
| 观测分子量 | 150-160 kDa |
| GenBank蛋白编号 | BC028375 |
| 基因名称 | PDZD8 |
| Gene ID (NCBI) | 118987 |
| RRID | AB_2880110 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8NEN9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDZD8 (PDZ domain containing 8) is an SMP (synaptotagmin-like mitochondrial-lipid-binding) domain-containing protein belonging to the tubular lipid-binding protein (TULIP) superfamily of lipid transfer proteins (PMID: 37961523). PDZD8 is an integral ER transmembrane protein present at ER-mitochondria contacts (PMID: 29097544). It is involved in regulating ER-mitochondria associations, mitochondrial calcium ion homeostasis, and lipid transport between the ER and late endosomes/lysosomes (PMID: 37247681; 29097544; 33912962).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PDZD8 antibody 25512-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
ACS Synth Biol Development of a Signal-integrating Reporter to Monitor Mitochondria-ER Contacts | ||
Nat Commun ER-mitochondria contacts mediate lipid radical transfer via RMDN3/PTPIP51 phosphorylation to reduce mitochondrial oxidative stress |