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验证数据展示

  • WB analysis using 21084-1-AP

    WB analysis using 21084-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21084-1-AP (PDZD7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse eye using 21084-1-AP

    IHC staining of mouse eye using 21084-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 21084-1-AP (PDZD7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse eye using 21084-1-AP

    IHC staining of mouse eye using 21084-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 21084-1-AP (PDZD7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PDZD7 Polyclonal antibody

PDZD7 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21084-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

PDZK7, PDZ domain-containing protein 7, PDZ domain containing 7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse ear tissue, mouse retina tissue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21084-1-AP targets PDZD7 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag15318

Product name: Recombinant human PDZD7 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-319 aa of BC029054

Sequence: MAQGFAVGFDPLGLGDLSSGSLSSLSSRGHLGSDSGSTATRYLLRKQQRLLNGPPRGIRASSPMGRVILINSPIEANSDESDIIHSVRVEKSPAGRLGFSVRGGSEHGLGIFVSKVEEGSSAERAGLCVGDKITEVNGLSLESTTMGSAVKVLTSSSRLHMMVRRMGRVPGIKFSKEKTTWVDVVNRRLVVEKCGSTPSDTSSEDGVRRIVHLYTTSDDFCLGFNIRGGKEFGLGIYVSKVDHGGLAEENGIKVGDQVLAANGVRFDDISHSQAVEVLKGQTHIMLTIKETGRYPAYKEMVSEYCWLDRLSNGVLQQLS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PDZ domain containing 7
别名 PDZK7, PDZ domain-containing protein 7, PDZ domain containing 7
计算分子量 517 aa, 56 kDa
观测分子量55 kDa
GenBank蛋白编号BC029054
基因名称 PDZD7
Gene ID (NCBI) 79955
RRIDAB_3085636
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H5P4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDZD7, also known as PDZK7, is essential in organizing the USH2 complex at stereocilia ankle links and blocks inhibition of adenylate cyclase activity mediated by ADGRV1. PDZD7 encodes a PDZ domain-containing scaffold protein that is highly expressed in hair cells of the inner ear. PDZD7 is also expressed in the cilia of photoreceptors (PMID: 33530996). Dysfunction or mutations of PDZD7 result in Usher syndrome type 2 (USH2) and Autosomal recessive non-syndromic hearing loss(ARNSHL) (PMID: 29048736, 20440071).

实验方案

Product Specific Protocols
WB protocol for PDZD7 antibody 21084-1-APDownload protocol
IHC protocol for PDZD7 antibody 21084-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols