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验证数据展示

  • WB analysis of rat kidney using 18795-1-AP

    WB analysis of rat kidney using 18795-1-AP

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 18795-1-AP (PDZD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse kidney using 18795-1-AP

    IP experiment of mouse kidney using 18795-1-AP

    IP result of anti-PDZD3 (IP:18795-1-AP, 4ug; Detection:18795-1-AP 1:500) with mouse kidney tissue lysate 4800ug.

PDZD3 Polyclonal antibody

PDZD3 Polyclonal Antibody for WB, IP, ELISA
Cat No. 18795-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

IKEPP, NaPi Cap2, NHERF 4, NHERF4, PDZ domain containing 3, PDZD3, PDZK2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat kidney tissue, mouse colon tissue
Positive IP detected inmouse kidney tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18795-1-AP targets PDZD3 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6931

Product name: Recombinant human PDZD3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 142-491 aa of BC029042

Sequence: DAHLCPTLGPGVRPRLCHIVKDEGGFGFSVTHGNQGPFWLVLSTGGAAERAGVPPGARLLEVNGLWQSGQQVTLLVAGPEVEEQCRQLGLPLAAPLAEGWALPTKPRCLHLEKGPQGFGFLLREEKGLDGRPGQFLWEVDPGLPAKKAGMQAGDRLVAVAGESVEGLGHEETVSRIQGQGSCVSLTVVDPEADRFFSMVRLSPLLFLENTEAPASPQGSSSASLVETEDPSLEDTSVPSVPLGSRQCFLYPGPGGSYGFRLSCVASGPRLFISQVTPGGSAARAGLQVGDVILEVNGYPVGGQNDLERLQQLPEAEPPLCLKLAARSLRGLEAWIPPGAAEDWALASDLL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PDZ domain containing 3
别名 IKEPP, NaPi Cap2, NHERF 4, NHERF4, PDZ domain containing 3, PDZD3, PDZK2
计算分子量 571 aa, 61 kDa
观测分子量 54-60 kDa
GenBank蛋白编号BC029042
基因名称 PDZD3
Gene ID (NCBI) 79849
RRIDAB_2162688
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86UT5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PDZD3 antibody 18795-1-APDownload protocol
IP protocol for PDZD3 antibody 18795-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols