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PDZ-GEF2 Polyclonal antibody

PDZ-GEF2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 24583-1-AP

Print datasheet

Synonyms

KIA001LB, PDZ GEF2, PDZGEF2, PDZ-GEF2, RA GEF 2, RAGEF2, RAPGEF6

验证数据展示

  • WB analysis using 24583-1-AP

    WB analysis using 24583-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24583-1-AP (PDZ-GEF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 24583-1-AP

    IHC staining of mouse brain using 24583-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24583-1-AP (PDZ-GEF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 24583-1-AP

    IHC staining of mouse brain using 24583-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24583-1-AP (PDZ-GEF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, mouse brain tissue, HeLa cells
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24583-1-AP targets PDZ-GEF2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen PDZ-GEF2 fusion protein Ag20325 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Rap guanine nucleotide exchange factor (GEF) 6
Synonyms KIA001LB, PDZ GEF2, PDZGEF2, PDZ-GEF2, RA GEF 2, RAGEF2, RAPGEF6
Calculated Molecular Weight 1601 aa, 179 kDa
Observed Molecular Weight179 kDa
GenBank Accession NumberBC142964
Gene Symbol RAPGEF6
Gene ID (NCBI) 51735
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TEU7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAPGEF6, also named PDZ-GEF2 enables several functions, including GTP-dependent protein binding activity; guanyl-nucleotide exchange factor activity; and phosphatidic acid binding activity. It is also involved in microvillus assembly; positive regulation of GTPase activity; and protein localization to plasma membrane. Located in several cellular components, including apical plasma membrane; centrosome; and endocytic vesicle.

实验方案

Product Specific Protocols
WB protocol for PDZ-GEF2 antibody 24583-1-APDownload protocol
IHC protocol for PDZ-GEF2 antibody 24583-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols