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验证数据展示

  • WB analysis using 24583-1-AP

    WB analysis using 24583-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24583-1-AP (PDZ-GEF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 24583-1-AP

    IHC staining of mouse brain using 24583-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24583-1-AP (PDZ-GEF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 24583-1-AP

    IHC staining of mouse brain using 24583-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24583-1-AP (PDZ-GEF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PDZ-GEF2 Polyclonal antibody

PDZ-GEF2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24583-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

KIA001LB, PDZ GEF2, PDZGEF2, PDZ-GEF2, RA GEF 2, RAGEF2, RAPGEF6

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse brain tissue, HeLa cells
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24583-1-AP targets PDZ-GEF2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag20325

Product name: Recombinant human PDZ-GEF2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-164 aa of BC142964

Sequence: MNSPVDPGARQALRKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQVLFCSETIARCWYILLSGSVLVKGSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENAKDNEDSILQREIPARQSRRRFRKINYKGERQTITDDVEVNSYL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Rap guanine nucleotide exchange factor (GEF) 6
别名 KIA001LB, PDZ GEF2, PDZGEF2, PDZ-GEF2, RA GEF 2, RAGEF2, RAPGEF6
计算分子量 1601 aa, 179 kDa
观测分子量179 kDa
GenBank蛋白编号BC142964
基因名称 RAPGEF6
Gene ID (NCBI) 51735
RRIDAB_3085740
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TEU7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAPGEF6, also named PDZ-GEF2 enables several functions, including GTP-dependent protein binding activity; guanyl-nucleotide exchange factor activity; and phosphatidic acid binding activity. It is also involved in microvillus assembly; positive regulation of GTPase activity; and protein localization to plasma membrane. Located in several cellular components, including apical plasma membrane; centrosome; and endocytic vesicle.

实验方案

Product Specific Protocols
IHC protocol for PDZ-GEF2 antibody 24583-1-APDownload protocol
WB protocol for PDZ-GEF2 antibody 24583-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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