验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Neuro-2a cells, mouse brain tissue |
| Positive IHC detected in | human liver tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
27490-1-AP targets PDXP in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | Human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | PDXP fusion protein Ag26592 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pyridoxal (pyridoxine, vitamin B6) phosphatase |
| 别名 | CIN, PDXP, PLP, PLP phosphatase, PLPP |
| 计算分子量 | 32 kDa |
| 观测分子量 | 32 kDa |
| GenBank蛋白编号 | BC064922 |
| 基因名称 | PDXP |
| Gene ID (NCBI) | 57026 |
| RRID | AB_2880887 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96GD0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDXP, also named CIN, belongs to the HAD-like hydrolase superfamily. As a pyridoxal phosphate (PLP) phosphatase, PDXP can catalyzes the dephosphorylation of pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP), with order of substrate preference PLP > PNP > PMP and therefore plays a role in vitamin B6 metabolism. PDXP is highly expressed in all the regions of central nerve system except the spinal cord. PDXP is also expressed at high level in liver and testis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PDXP antibody 27490-1-AP | Download protocol |
| IHC protocol for PDXP antibody 27490-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
