验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, L02 cells, mouse liver tissue, mouse spinal cord, rat liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human urothelial carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15309-1-AP targets PDXK in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag7451 Product name: Recombinant human PDXK protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-312 aa of BC000123 Sequence: MEEECRVLSIQSHVIRGYVGNRAATFPLQVLGFEIDAVNSVQFSNHTGYAHWKGQVLNSDELQELYEGLRLNNMNKYDYVLTGYTRDKSFLAMVVDIVQELKQQNPRLVYVCDPVLGDKWDGEGSMYVPEDLLPVYKEKVVPLADIITPNQFEAELLSGRKIHSQEEALRVMDMLHSMGPDTVVITSSDLPSPQGSNYLIVLGSQRRRNPAGSVVMERIRMDIRKVDAVFVGTGDLFAAMLLAWTHKHPNNLKVACEKTVSTLHHVLQRTIQCAKAQAGEGVRPSPMQLELRMVQSKRDIEDPEIVVQATVL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pyridoxal (pyridoxine, vitamin B6) kinase |
| 别名 | C21orf124, C21orf97, PDXK, PKH, PNK, PRED79, Pyridoxal kinase, Pyridoxine kinase |
| 计算分子量 | 35 kDa |
| 观测分子量 | 40 kDa, 35 kDa |
| GenBank蛋白编号 | BC000123 |
| 基因名称 | PDXK |
| Gene ID (NCBI) | 8566 |
| RRID | AB_2252232 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O00764 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDXK encodes for a pyridoxal kinase, which converts inactive B6 vitamers to the active cofactor pyridoxal 5'-phosphate (PLP).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PDXK antibody 15309-1-AP | Download protocol |
| IP protocol for PDXK antibody 15309-1-AP | Download protocol |
| WB protocol for PDXK antibody 15309-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Pharmacol A pan-cancer analysis of the expression and molecular mechanism of DHX9 in human cancers | ||
Cell Rep Integrative multi-omic profiling of adult mouse brain endothelial cells and potential implications in Alzheimer's disease | ||
Drug Deliv Transl Res Delivery of exogenous miR-19b by Wharton's Jelly Mesenchymal Stem Cells attenuates transplanted kidney ischemia/reperfusion injury by regulating cellular metabolism | ||
Nat Commun SLC25A38 is required for mitochondrial pyridoxal 5'-phosphate (PLP) accumulation |
