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验证数据展示

  • WB analysis using 67520-1-Ig

    WB analysis using 67520-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67520-1-Ig (PDS5A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67520-1-Ig

    IHC staining of human breast cancer using 67520-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67520-1-Ig (PDS5A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PDS5A antibody included
  • WB analysis of HeLa using 67520-1-Ig

    WB analysis of HeLa using 67520-1-Ig

    WB result of PDS5A antibody (67520-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDS5A transfected HeLa cells.

  • IF Staining of A431 using 67520-1-Ig

    IF Staining of A431 using 67520-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using PDS5A antibody (67520-1-Ig, Clone: 1C8D4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • IF Staining of HepG2 using 67520-1-Ig

    IF Staining of HepG2 using 67520-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PDS5A antibody (67520-1-Ig, Clone: 1C8D4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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PDS5A Monoclonal antibody

PDS5A Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67520-1-Ig

产品说明书

CloneNo. 1C8D4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

1C8D4, Cell proliferation-inducing gene 54 protein, KIAA0648, PDS5, PIG54

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PDS5A Ready-To-Use IHC Kit. PDS5A primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67520-1-Ig targets PDS5A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag11525

Product name: Recombinant human PDS5A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2-378 aa of BC009650

Sequence: VKERRAHARQCLLKNISIRREYIKQNPMATEKLLSLLPEYVVPYMIHLLAHDPDFTRSQDVDQLRDIKECLWFMLEVLMTKNENNSHAFMKKMAENIKLTRDAQSPDESKTNEKLYTVCDVALCVINSKSALCNADSPKDPVLPMKFFTQPEKDFCNDKSYISEETRVLLLTGKPKPAGVLGAVNKPLSATGRKPYVRSTGTETGSNINVNSELNPSTGNRSREQSSEAAETGVSENEENPVRIISVTPVKNIDPVKNKEINSDQATQGNISSDRGKKRTVTAAGAENIQQKTDEKVDESGPPAPSKPRRGRRPKSESQGNATKNDDLNKPINKGRKRAAVGQESPGGLEAGNAKAPKLQDLAKKAAPAERQIDLQR

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 PDS5, regulator of cohesion maintenance, homolog A (S. cerevisiae)
别名 1C8D4, Cell proliferation-inducing gene 54 protein, KIAA0648, PDS5, PIG54
计算分子量 1297 aa, 147 kDa
观测分子量130 kDa
GenBank蛋白编号BC009650
基因名称 PDS5A
Gene ID (NCBI) 23244
RRIDAB_2882740
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ29RF7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PDS5A antibody 67520-1-IgDownload protocol
IHC protocol for PDS5A antibody 67520-1-IgDownload protocol
IF protocol for PDS5A antibody 67520-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols