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验证数据展示

  • WB analysis of human heart using 13199-2-AP (same clone as 13199-2-PBS)

    WB analysis of human heart using 13199-2-AP (same clone as 13199-2-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 13199-2-AP (PDLIM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13199-2-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 13199-2-AP (same clone as 13199-2-PBS)

    IHC staining of human stomach cancer using 13199-2-AP (same clone as 13199-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13199-2-AP (PDLIM3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13199-2-PBS in a different storage buffer formulation.

PDLIM3 Polyclonal antibody, PBS Only

PDLIM3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 13199-2-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

ALP, Actinin associated LIM protein, Actinin-associated LIM protein, Alpha-actinin-2-associated LIM protein, PDZ and LIM domain 3

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

13199-2-PBS targets PDLIM3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag3934

Product name: Recombinant human PDLIM3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-316 aa of BC027870

Sequence: MPQTVILPGPAPWGFRLSGGIDFNQPLVITRITPGSKAAAANLCPGDVILAIDGFGTESMTHADAQDRIKAAAHQLCLKIDRGETHLWSPQVSEDGKAHPFKINLESEPQEFKPIGTAHNRRAQPFVAAANIDDKRQVVSASYNSPIGLYSTSNIQDALHGQLRGLIPSSPQNEPTASVPPESDVYRMLHDNRNEPTQPRQSGSFRVLQGMVDDGSDDRPAGTRSVRAPVTKVHGGSGGAQRMPLCDKCGSGIVGAVVKARDKYRHPECFVCADCNLNLKQKGYFFIEGELYCETHARARTKPPEGYDTVTLYPKA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PDZ and LIM domain 3
别名 ALP, Actinin associated LIM protein, Actinin-associated LIM protein, Alpha-actinin-2-associated LIM protein, PDZ and LIM domain 3
计算分子量 316 aa, 34 kDa
观测分子量 39 kDa
GenBank蛋白编号BC027870
基因名称 PDLIM3
Gene ID (NCBI) 27295
RRIDAB_2161653
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ53GG5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PDLIM3, also known as ALP, contains a PDZ domain and a LIM domain. PDLIM3 binds the spectrin-like repeats of alpha-actinin-2 and colocalizes with alpha-actinin-2 at the Z lines of skeletal muscle.

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