验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse heart tissue, rat brain tissue, mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human heart tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 8 publications below |
| IHC | See 4 publications below |
产品信息
12215-1-AP targets PDK3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag2879 Product name: Recombinant human PDK3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 129-369 aa of BC015948 Sequence: GVIEYKEKFGFDPFISTNIQYFLDRFYTNRISFRMLINQHTLLFGGDTNPVHPKHIGSIDPTCNVADVVKDAYETAKMLCEQYYLVAPELEVEEFNAKAPDKPIQVVYVPSHLFHMLFELFKNSMRATVELYEDRKEGYPAVKTLVTLGKEDLSIKISDLGGGVPLRKIDRLFNYMYSTAPRPSLEPTRAAPLAGFGYGLPISRLYARYFQGDLKLYSMEGVGTDAVIYLKALSSESFERL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pyruvate dehydrogenase kinase, isozyme 3 |
| 别名 | [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial, CMTX6, EC:2.7.11.2, GS1-358P8.4, PDHK3 |
| 计算分子量 | 406 aa, 47 kDa |
| 观测分子量 | 47 kDa |
| GenBank蛋白编号 | BC015948 |
| 基因名称 | PDK3 |
| Gene ID (NCBI) | 5165 |
| RRID | AB_2877836 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15120 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDK3([Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3, mitochondrial) is also named as PDHK3 and belongs to the PDK/BCKDK protein kinase family. It inhibits pyruvate dehydrogenase activity by phosphorylation of the E1 subunit PDHA1, and thereby regulates glucose metabolism and aerobic respiration. It has 2 isoforms produced by alternative splicing with the molecular weight of 47 and 48 kDa. This protein can exsit as a dimer with the molecular weight of 90 kDa(PMID:11978179).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PDK3 antibody 12215-1-AP | Download protocol |
| IP protocol for PDK3 antibody 12215-1-AP | Download protocol |
| WB protocol for PDK3 antibody 12215-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun NOTCH1 mitochondria localization during heart development promotes mitochondrial metabolism and the endothelial-to-mesenchymal transition in mice | ||
Nat Commun Mitophagy mitigates mitochondrial fatty acid β-oxidation deficient cardiomyopathy. | ||
Nat Commun PDK4 drives abdominal aortic aneurysm by promoting smooth muscle cell metabolic reprogramming and NLRP3-mediated pyroptosis. | ||
Mater Today Bio Dual activation of cuproptosis and excessive autophagy by copper-bismuth metal-organic frameworks-loaded detachable microneedles in oral leukoplakia. | ||
Environ Pollut Acrolein exposure affects ovarian function by interfering with glycolysis and mitochondrial energy metabolism in mouse |
