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验证数据展示

  • WB analysis using 26305-1-AP

    WB analysis using 26305-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26305-1-AP (PDILT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours; mouse brain tissue, mouse heart tissue and mouse lung tissue are negative control.

  • IF Staining of mouse testis using 26305-1-AP

    IF Staining of mouse testis using 26305-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 26305-1-AP (PDILT antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human testis using 26305-1-AP

    IHC staining of human testis using 26305-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26305-1-AP (PDILT Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human testis using 26305-1-AP

    IHC staining of human testis using 26305-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26305-1-AP (PDILT Antibody) at dilution of 1:200 (under 40x lens).

PDILT Polyclonal antibody

PDILT Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 26305-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

PDIA7, PDILT

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26305-1-AP targets PDILT in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PDILT fusion protein Ag23675 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein disulfide isomerase-like, testis expressed
别名 PDIA7, PDILT
观测分子量 67 kDa, 75 kDa
GenBank蛋白编号BC042607
基因名称 PDILT
Gene ID (NCBI) 204474
RRIDAB_2880472
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N807
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDILT, a divergent testis-specific protein disulfide isomerase with a non-classical SXXC motif that engages in disulfide-dependent interactions in the endoplasmic reticulum (PMID: 15475357). PDILT interacts with CALR3 in the testicular germ cells and plays an indispensable role in the disulfide formation in the ADAM3. The PDILT knockout mice were male infertile, consistent with the previous observations that spermatozoa lacking ADAM3 cannot migrate through the UTJ and cannot bind to the ZP (PMID: 22357757). It can be detected as 67 kDa and 75kDa due to the glycosylation.

实验方案

Product Specific Protocols
WB protocol for PDILT antibody 26305-1-APDownload protocol
IHC protocol for PDILT antibody 26305-1-APDownload protocol
IF protocol for PDILT antibody 26305-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols