验证数据展示
经过测试的应用
| Positive WB detected in | rat kidney tissue, mouse heart tissue, mouse liver tissue, mouse kidney tissue, mouse lung tissue, HeLa cells, L02 cells, HEK-293 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | mouse brain tissue, human spleen tissue, human stomach tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy PDIA6 Ready-To-Use IHC Kit. PDIA6 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18233-1-AP targets PDIA6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | PDIA6 fusion protein Ag12940 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein disulfide isomerase family A, member 6 |
| 别名 | Protein disulfide isomerase P5, Protein disulfide isomerase A6, P5, ERp5, ER protein 5 |
| 计算分子量 | 48 kDa, 54 kDa |
| 观测分子量 | 48-54 kDa |
| GenBank蛋白编号 | BC001312 |
| 基因名称 | PDIA6 |
| Gene ID (NCBI) | 10130 |
| RRID | AB_10805765 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15084 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDIA6(Protein disulfide-isomerase A6) is also named as ERP5, P5, TXNDC7 and belongs to the protein disulfide isomerase family.It is one of the endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins(PMID:7590364).It has 2 isoforms produced by alternative splicing.This antibody is specific to PDIA6.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PDIA6 antibody 18233-1-AP | Download protocol |
| IHC protocol for PDIA6 antibody 18233-1-AP | Download protocol |
| IF protocol for PDIA6 antibody 18233-1-AP | Download protocol |
| IP protocol for PDIA6 antibody 18233-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Cell p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors. | ||
Cell Res Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2. | ||
J Exp Med Essential cell-extrinsic requirement for PDIA6 in lymphoid and myeloid development. | ||
Nucleic Acids Res Melanoma RBPome identification reveals PDIA6 as an unconventional RNA-binding protein involved in metastasis.
| ||
J Immunother Cancer MUC1-C is a master regulator of MICA/B NKG2D ligand and exosome secretion in human cancer cells | ||
Antiviral Res Venezuelan equine encephalitis virus E1 protein interacts with PDIA6 and PDI inhibition reduces alphavirus production |
