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验证数据展示

  • WB analysis using 10951-1-AP (same clone as 10951-1-PBS)

    WB analysis using 10951-1-AP (same clone as 10951-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10951-1-AP (PDHX antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10951-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 10951-1-AP (same clone as 10951-1-PBS)

    IHC staining of human colon cancer using 10951-1-AP (same clone as 10951-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 10951-1-AP (PDHX antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10951-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PDHX antibody included
  • WB analysis using 10951-1-AP (same clone as 10951-1-PBS)

    WB analysis using 10951-1-AP (same clone as 10951-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10951-1-AP (PDHX antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10951-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 10951-1-AP (same clone as 10951-1-PBS)

    IP experiment of HEK-293 using 10951-1-AP (same clone as 10951-1-PBS)

    IP result of anti-PDHX (IP:10951-1-AP, 4ug; Detection:10951-1-AP 1:5000) with HEK-293 cells lysate 2000ug. This data was developed using the same antibody clone with 10951-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PDHX Polyclonal antibody, PBS Only

PDHX Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 10951-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

PDX1, pyruvate dehydrogenase complex, component X, Lipoyl-containing pyruvate dehydrogenase complex component X, E3-binding protein, E3 binding protein

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PDHX Ready-To-Use IHC Kit. PDHX primary antibody included.

产品信息

10951-1-PBS targets PDHX in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1389

Product name: Recombinant human PDHX protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-501 aa of BC010389

Sequence: MAASWRLGCDPRLLRYLVGFPGRRSVGLVKGALGWSVSRGANWRWFHSTQWLRGDPIKILMPSLSPTMEEGNIVKWLKKEGEAVSAGDALCEIETDKAVVTLDASDDGILAKIVVEEGSKNIRLGSLIGLIVEEGEDWKHVEIPKDVGPPPPVSKPSEPRPSPEPQISIPVKKEHIPGTLRFRLSPAARNILEKHSLDASQGTATGPRGIFTKEDALKLVQLKQTGKITESRPTPAPTATPTAPSPLQATAGPSYPRPVIPPVSTPGQPNAVGTFTEIPASNIRRVIAKRLTESKSTVPHAYATADCDLGAVLKVRQDLVKDDIKVSVNDFIIKAAAVTLKQMPDVNVSWDGEGPKQLPFIDISVAVATVKGLLTPIIKDAAAKGIQEIADSVKALSKKARDGKLLPEEYQGGSFSISNLGMFGIDEFTAVINPPQACILAVGRFRPVLKLTEDEEGNAKLQQRQLITVTMSSDSRVVDDELATRFLKSFKANLENPIRLA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pyruvate dehydrogenase complex, component X
别名 PDX1, pyruvate dehydrogenase complex, component X, Lipoyl-containing pyruvate dehydrogenase complex component X, E3-binding protein, E3 binding protein
计算分子量 54 kDa
观测分子量 54 kDa
GenBank蛋白编号BC010389
基因名称 PDHX
Gene ID (NCBI) 8050
RRIDAB_2163102
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00330
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PDHX (Pyruvate dehydrogenase protein X component), an E3-binding protein in the PDC, is acetylated by the p300 at Lys 488, impeding the interaction between PDHX and dihydrolipoyl transacetylase (E2), thereby disrupting PDC assembly to inhibit its activation (PMID: 30012170). PDHX was characterized as a metabolically essential gene for esophageal squamous cell carcinoma (ESCC), and knockdown of PDHX inhibited the proliferation of cancer stem cells (CSCs) and in vivo tumor growth (PMID: 33964039). Western blot analysis detected PDHX at an apparent molecular mass of 54 kDa.

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