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验证数据展示

  • WB analysis using 68238-1-Ig (same clone as 68238-1-PBS)

    WB analysis using 68238-1-Ig (same clone as 68238-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68238-1-Ig (PDHB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68238-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 68238-1-Ig (same clone as 68238-1-PBS)

    IHC staining of mouse heart using 68238-1-Ig (same clone as 68238-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68238-1-Ig (PDHB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68238-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PDHB antibody included
  • IF Staining of HeLa using 68238-1-Ig (same clone as 68238-1-PBS)

    IF Staining of HeLa using 68238-1-Ig (same clone as 68238-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PDHB antibody (68238-1-Ig, Clone: 2A7C10 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68238-1-PBS in a different storage buffer formulation.

PDHB Monoclonal antibody, PBS Only

PDHB Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68238-1-PBS

产品说明书

CloneNo. 2A7C10

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

2A7C10, EC:1.2.4.1, PDHE1B, PDHE1-B, PHE1B

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PDHB Ready-To-Use IHC Kit. PDHB primary antibody included.

产品信息

68238-1-PBS targets PDHB in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag6857

Product name: Recombinant human PDHB protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-359 aa of BC000439

Sequence: MAAVSGLVRRPLREVSGLLKRRFHWTAPAALQVTVRDAINQGMDEELERDEKVFLLGEEVAQYDGAYKVSRGLWKKYGDKRIIDTPISEMGFAGIAVGAAMAGLRPICEFMTFNFSMQAIDQVINSAAKTYYMSGGLQPVPIVFRGPNGASAGVAAQHSQCFAAWYGHCPGLKVVSPWNSEDAKGLIKSAIRDNNPVVVLENELMYGVPFEFPPEAQSKDFLIPIGKAKIERQGTHITVVSHSRPVGHCLEAAAVLSKEGVECEVINMRTIRPMDMETIEASVMKTNHLVTVEGGWPQFGVGAEICARIMEGPAFNFLDAPAVRVTGADVPMPYAKILEDNSIPQVKDIIFAIKKTLNI

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 pyruvate dehydrogenase (lipoamide) beta
别名 2A7C10, EC:1.2.4.1, PDHE1B, PDHE1-B, PHE1B
计算分子量 39 kDa
观测分子量 34 kDa
GenBank蛋白编号BC000439
基因名称 PDHB
Gene ID (NCBI) 5162
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP11177
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PDHB (Pyruvate dehydrogenase E1 component subunit beta, mitochondrial) is also named PHE1B. The enzyme, which is found in mitochondria, is one of the component enzymes of the pyruvate dehydrogenase multienzyme complex (PDH). It catalyzes the first reaction of an oxidative decarboxylation sequence converting pyruvate to acetyl-CoA and CO(2)(PMID:3422424). Defects in PDHB are the cause of pyruvate dehydrogenase E1-beta deficiency (PDHBD)(PMID:15138885).

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