- Featured Product
- KD/KO Validated
PDH E1 Alpha Polyclonal antibody
PDH E1 Alpha Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
IF, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK293 cells, mouse brain tissue, rat brain tissue, rat kidney tissue |
Positive IP detected in | mouse kidney tissue |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
产品信息
18068-1-AP targets PDH E1 Alpha in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF, IHC, IP, WB, ELISA |
Cited Applications | IF, IHC, IP, WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, chicken |
Immunogen | PDH E1 Alpha fusion protein Ag12701 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | pyruvate dehydrogenase (lipoamide) alpha 1 |
Synonyms | PDH E1 alpha, PDH E1α, PDHA1, PDHE1 A type I, pyruvate dehydrogenase E1 α subunit |
Calculated molecular weight | 43 kDa |
Observed molecular weight | 39-48 kDa |
GenBank accession number | BC002406 |
Gene symbol | PDHA1 |
Uniprot | P08559 |
Gene ID (NCBI) | 5160 |
RRID | AB_2162931 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for PDH E1 Alpha antibody 18068-1-AP | Download protocol |
IHC protocol for PDH E1 Alpha antibody 18068-1-AP | Download protocol |
IF protocol for PDH E1 Alpha antibody 18068-1-AP | Download protocol |
IP protocol for PDH E1 Alpha antibody 18068-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Cell Biol RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis
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Cell Stem Cell A small-molecule cocktail promotes mammalian cardiomyocyte proliferation and heart regeneration. | ||
Cell Metab Environment Impacts the Metabolic Dependencies of Ras-Driven Non-Small Cell Lung Cancer.
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Mol Cell Asparagine plays a critical role in regulating cellular adaptation to glutamine depletion.
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Circ Res (Pro)renin Receptor Inhibition Reprograms Hepatic Lipid Metabolism and Protects Mice from Diet-Induced Obesity and Hepatosteatosis. | ||
Nat Commun A shift in glutamine nitrogen metabolism contributes to the malignant progression of cancer. |