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验证数据展示

  • WB analysis using 60535-13-Ig

    WB analysis using 60535-13-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60535-13-Ig (PDGFR beta/CD140b antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of U2OS using 60535-13-Ig

    WB analysis of U2OS using 60535-13-Ig

    U2OS cells were subjected to SDS PAGE followed by western blot with 60535-13-Ig (PDGFR beta/CD140b antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

PDGFR beta/CD140b Monoclonal antibody

PDGFR beta/CD140b Monoclonal Antibody for WB, ELISA
Cat No. 60535-13-Ig

产品说明书

CloneNo. 4E2D5

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, ELISA

别名

PDGFRB, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, CD140B

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSH-SY5Y cells, U2OS cells, SK-N-SH cells
Planning an IHC experiment? We recommend our IHCeasy PDGFRB Ready-To-Use IHC Kit. PDGFRB primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60535-13-Ig targets PDGFR beta/CD140b in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg0147

Product name: Recombinant Human PDGFR beta/CD140b protein (Myc Tag, His Tag)

Source: mammalian cells-derived, pHZ-KIsec

Tag: Myc & 6*His

Domain: 33-531 aa of BC032224

Sequence: LVVTPPGPELVLNVSSTFVLTCSGSAPVVWERMSQEPPQEMAKAQDGTFSSVLTLTNLTGLDTGEYFCTHNDSRGLETDERKRLYIFVPDPTVGFLPNDAEELFIFLTEITEITIPCRVTDPQLVVTLHEKKGDVALPVPYDHQRGFFGIFEDRSYICKTTIGDREVDSDAYYVYRLQVSSINVSVNAVQTVVRQGENITLMCIVIGNEVVNFEWTYPRKESGRLVEPVTDFLLDMPYHIRSILHIPSAELEDSGTYTCNVTESVNDHQDEKAINITVVESGYVRLLGEVGTLQFAELHRSRTLQVVFEAYPPPTVLWFKDNRTLGDSSAGEIALSTRNVSETRYVSELTLVRVKVAEAGHYTMRAFHEDAEVQLSFQLQINVPVRVLELSESHPDSGEQTVRCRGRGMPQPNIIWSACRDLKRCPRELPPTLLGNSSEEESQLETNVTYWEEEQEFEVVSTLRLQHVDRPLSVRCTLRNAVGQDTQEVIVVPHSLPFK

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 platelet-derived growth factor receptor, beta polypeptide
别名 PDGFRB, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, CD140B
计算分子量 1106 aa, 124 kDa
观测分子量160-180 kDa
GenBank蛋白编号BC032224
基因名称 PDGFR beta
Gene ID (NCBI) 5159
RRIDAB_3743219
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP09619
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDGFRs are receptor tyrosine kinases with an extracellular ligand-binding domain and a cytoplasmic enzymatic domain. The signaling activity of PDGFRs is tightly regulated by an autoinhibitory allosteric conformation, and requires ligand-induced receptor dimerization at the cell surface to alleviate autoinhibition and achieve physiological activity (PMID: 21664579). PDGFR beta (platelet-derived growth factor receptor- β)/CD140b is a constitutive marker expressed by ASCs in the angiogenic behavior of human retinal endothelial cells (HREs). CD140b (PDGFRβ) plays a crucial role in controlling the intrinsic proliferation (PMID: 30976657)

实验方案

Product Specific Protocols
WB protocol for PDGFR beta/CD140b antibody 60535-13-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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