验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, human heart tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
81718-2-RR targets PDGF-D in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Eg6204 Product name: Recombinant human PDGFD protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 19-370 aa of NM_025208.4 Sequence: RDTSATPQSASIKALRNANLRRDESNHLTDLYRRDETIQVKGNGYVQSPRFPNSYPRNLLLTWRLHSQENTRIQLVFDNQFGLEEAENDICRYDFVEVEDISETSTIIRGRWCGHKEVPPRIKSRTNQIKITFKSDDYFVAKPGFKIYYSLLEDFQPAAASETNWESVTSSISGVSYNSPSVTDPTLIADALDKKIAEFDTVEDLLKYFNPESWQEDLENMYLDTPRYRGRSYHDRKSKVDLDRLNDDAKRYSCTPRNYSVNIREELKLANVVFFPRCLLVQRCGGNCGCGTVNWRSCTCNSGKTVKKYHEVLQFEPGHIKRRGRAKTMALVDIQLDHHERCDCICSSRPPR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | platelet derived growth factor D |
| 别名 | PDGFD, Iris-expressed growth factor, PDGF D, PDGFD receptor binding form, Platelet-derived growth factor D |
| 计算分子量 | 43 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | NM_025208.4 |
| 基因名称 | PDGFD |
| Gene ID (NCBI) | 80310 |
| RRID | AB_3743391 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9GZP0-1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Platelet-derived growth factor-D (PDGF-D) is the newest member of the PDGF family. It is secreted as a latent dimer that must undergo extracellular proteolytic removal of its N-terminal CUB domain before the growth-factor domain can activate the β-PDGFR. Once activated, PDGF-D drives proliferation, migration and survival of mesenchymal cells and promotes angiogenesis, wound healing and tissue remodeling. Clinically, PDGF-D is up-regulated in several cancers and in fibrotic diseases of the heart, kidney and lung, making it a potential therapeutic target.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PDGF-D antibody 81718-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
