验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, mouse kidney tissue, rat kidney tissue |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
26940-1-AP targets PDGFA in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | PDGFA fusion protein Ag25643 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | platelet-derived growth factor alpha polypeptide |
别名 | PDGF-1, PDGF1, PDGF subunit A, PDGF AA, PDGF A |
计算分子量 | 196 aa, 22 kDa |
观测分子量 | 13-16 kDa |
GenBank蛋白编号 | BC146594 |
基因名称 | PDGFA |
Gene ID (NCBI) | 5154 |
RRID | AB_3669576 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P04085 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDGFA is a member of the platelet-derived growth factor family. Four members of this family are mitogenic factors for cells of mesenchymal origin, and they are characterized by a motif of eight cysteines. PDGFA plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, and chemotaxis. It is also required for normal lung alveolar septum formation during embryogenesis, development of the gastrointestinal tract, and spermatogenesis. PDGFA is also involved in oligodendrocyte development and the myelination process in the spinal cord and cerebellum. PDGF-A protein monomer is about 13-16 kDa, and PDGF-AA is a 28.5 kDa A chain homodimer.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for PDGFA antibody 26940-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |