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验证数据展示

  • WB analysis using 28573-1-AP

    WB analysis using 28573-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28573-1-AP (PDE6D antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 28573-1-AP

    WB analysis of HeLa using 28573-1-AP

    WB result of PDE6D antibody (28573-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDE6D transfected HeLa cells.

  • Featured Product
  • KD/KO验证

PDE6D Polyclonal antibody

PDE6D Polyclonal Antibody for WB, ELISA
Cat No. 28573-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

PDED

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, human placenta tissue, mouse brain tissue, rat brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28573-1-AP targets PDE6D in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag29563

Product name: Recombinant human PDE6D protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-150 aa of NM_002601

Sequence: MSAKDERAREILRGFKLNWMNLRDAETGKILWQGTEDLSVPGVEHEARVPKKILKCKAVSRELNFSSTEQMEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAAPESQMMPASVLTGNVIIETKFFDDDLLVSTSRVRLFYV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphodiesterase 6D, cGMP-specific, rod, delta
别名 PDED
计算分子量 17 KDa
观测分子量 14-17 kDa
GenBank蛋白编号NM_002601
基因名称 PDE6D
Gene ID (NCBI) 5147
RRIDAB_2881173
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43924
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDE6D, also known as PDED, belongs to the PDE6D/unc-119 family. PDE6D is a ubiquitously expressed prenyl-binding protein. By binding to prenyl moieties, PDE6D mediates the shuttling of prenylated proteins between membranes. Thereby, it regulates the membrane association of Ras and Rap GTPases (PMID: 36672247). In addition, research has shown that PDE6D is overexpressed in liver cancer and correlated with tumor stages, grading, and ERK activation (PMID: 30901922). The calculated molecular weight of PDE6D is 17 kDa.

实验方案

Product Specific Protocols
WB protocol for PDE6D antibody 28573-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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