验证数据展示
经过测试的应用
| Positive WB detected in | mouse eye tissue, A549 cells, rat eye tissue |
| Positive IP detected in | Y79 cells |
| Positive IHC detected in | rat eye tissue, mouse eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat eye tissue, mouse eye tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22063-1-AP targets PDE6B in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, zebrafish |
| 免疫原 |
CatNo: Ag17139 Product name: Recombinant human PDE6B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-88 aa of BC000249 Sequence: MSLSEEQARSFLDQNPDFARQYFGKKLSPENVAAACEDGCPPDCDSLRDLCQVEESTALLELVQDMQESINMERVVFKVLRRLCTLLQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phosphodiesterase 6B, cGMP-specific, rod, beta |
| 别名 | EC:3.1.4.35, GMP PDE beta, GMP-PDE beta, PDEB, Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta |
| 计算分子量 | 854 aa, 98 kDa |
| 观测分子量 | 98 kDa |
| GenBank蛋白编号 | BC000249 |
| 基因名称 | PDE6B |
| Gene ID (NCBI) | 5158 |
| RRID | AB_2619668 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P35913 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDE6 is a cGMP-specific PDE family and presents multicomponent enzyme complexes. The rod PDE6 enzyme is comprised of two catalytic subunits (PDE6A and PDE6B) and it is expressed in human lungs (PMID:20979602). PDE6B(Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta) is also named as PDEB and belongs to the cyclic nucleotide phosphodiesterase family. PDE6B also has a C-terminal CAAX motif for posttranslational processing involving lipidation, proteolysis, and carboxymethylation (PMID:8394243). Defects in PDE6B are the cause of retinitis pigmentosa type 40 (RP40)(PMID:22334370) and congenital stationary night blindness autosomal dominant type 2 (CSNBAD2) (PMID:8075643).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for PDE6B antibody 22063-1-AP | Download protocol |
| IHC protocol for PDE6B antibody 22063-1-AP | Download protocol |
| IP protocol for PDE6B antibody 22063-1-AP | Download protocol |
| WB protocol for PDE6B antibody 22063-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv Synergistic antioxidant and gene supplementation for high-efficacy retinitis pigmentosa therapy. | ||
Research (Wash D C) Precise Correction of the Pde6b-L659P Mutation Causing Retinal Degeneration with Minimum Bystander Editing by Advanced Genome Editing Tools | ||
Proc Natl Acad Sci U S A Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome. | ||
NPJ Parkinsons Dis Multi-omic insight into the molecular mechanism of cuproptosis-related genes in the pathogenesis of Parkinson's disease. | ||
Stem Cell Res Ther Retinoic acid delays initial photoreceptor differentiation and results in a highly structured mature retinal organoid | ||
Zool Res Tubulin hyperglutamylation induces retinal degeneration by disrupting ciliary architecture and protein trafficking. |
