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PDE4D Polyclonal antibody

PDE4D Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12918-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (1)

PDE43, EC:3.1.4.53, DPDE3, cAMP-specific phosphodiesterase 4D, 3',5'-cyclic-AMP phosphodiesterase 4D

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inK-562 cells, mouse brain tissue, mouse pancreas tissue
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12918-1-AP targets PDE4D in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, rabbit
免疫原 PDE4D fusion protein Ag3588 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila)
别名 PDE43, EC:3.1.4.53, DPDE3, cAMP-specific phosphodiesterase 4D, 3',5'-cyclic-AMP phosphodiesterase 4D
计算分子量 809 aa, 91 kDa
观测分子量 91 kDa
GenBank蛋白编号BC036319
基因名称 PDE4D
Gene ID (NCBI) 5144
RRIDAB_2161097
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ08499
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDE4D(cAMP-specific 3',5'-cyclic phosphodiesterase 4D) is also named as DPDE3, PDE43 and belongs to the cyclic nucleotide phosphodiesterase family and PDE4 subfamily. PDE4D subtype may be of particular importance as an antidepressant target in that it is regulated by repeated treatment with both norepinephrine and serotonin reuptake inhibitors as well as by the PDE4 inhibitor rolipram(PMID:16730340). It has some isoforms and all the PDE4D isoforms are expressed in healthy adult human hippocampus and in hippocampus from a patient with advanced Alzheimer disease(PMID:17353396).

实验方案

Product Specific Protocols
WB protocol for PDE4D antibody 12918-1-APDownload protocol
IHC protocol for PDE4D antibody 12918-1-APDownload protocol
IF protocol for PDE4D antibody 12918-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Metab

FGF1 and insulin control lipolysis by convergent pathways.

Authors - Gencer Sancar
humanWB

Acta Pharm Sin B

Targeting PDE4 as a promising therapeutic strategy in chronic ulcerative colitis through modulating mucosal homeostasis.

Authors - Heng Li
mouseWB

Cell Rep

miR-199a Links MeCP2 with mTOR Signaling and Its Dysregulation Leads to Rett Syndrome Phenotypes.

Authors - Keita Tsujimura
humanWB

JCI Insight

Inhibition of phosphodiesterase 4D suppresses mTORC1 signaling and pancreatic cancer growth

Authors - Mi-Hyeon Jeong
WB

Diabetes

Salt inducible kinase 1 terminates cAMP signaling by an evolutionarily conserved negative feedback loop in beta cells.

Authors - Min-Jung Kim
humanWB,IHC,IF

Cancer Lett

PDE4D binds and interacts with YAP to cooperatively promote HCC progression.

Authors - Huili Ren
  • KD Validated
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