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验证数据展示

  • WB analysis using 85996-1-RR

    WB analysis using 85996-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85996-1-RR (PDE3B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse adipose using 85996-1-RR

    IHC staining of mouse adipose using 85996-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse adipose tissue slide using 85996-1-RR (PDE3B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85996-1-RR

    Affinity and Kinetic Characterization of 85996-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85996-1-RR against Human PDE3B were performed. The affinity constant is below 1 pM.

PDE3B Recombinant antibody

PDE3B Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 85996-1-RR

产品说明书

CloneNo. 250460H7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

CGIP1, CGI-PDE B, cGIPDE1, cGMP-inhibited 3',5'-cyclic phosphodiesterase 3B, Cyclic GMP-inhibited phosphodiesterase B

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, mouse adipose tissue, Jurkat cells
Positive IHC detected inmouse adipose tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85996-1-RR targets PDE3B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 PDE3B fusion protein Ag19190 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 phosphodiesterase 3B, cGMP-inhibited
别名 CGIP1, CGI-PDE B, cGIPDE1, cGMP-inhibited 3',5'-cyclic phosphodiesterase 3B, Cyclic GMP-inhibited phosphodiesterase B
计算分子量 1112 aa, 124 kDa
观测分子量 124 kDa
GenBank蛋白编号BC136565
基因名称 PDE3B
Gene ID (NCBI) 5140
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13370
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDE3B is a cyclic nucleotide phosphodiesterase with dual specificity for cAMP and cGMP. It plays crucial roles in regulating physiological processes such as angiogenesis and cardiac contractility. PDE3B can be activated by insulin and is involved in the antilipolytic actions of insulin. Additionally, PDE3B forms macromolecular complexes that are important for its activation and interaction with other proteins (PMID: 22001403, PMID: 26031333).

实验方案

Product Specific Protocols
WB protocol for PDE3B antibody 85996-1-RRDownload protocol
IHC protocol for PDE3B antibody 85996-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols